Online ISSN: 2515-8260

Keywords : Phytochemicals


An integrated in silico approach to identify bioactive Phytochemicals in Bauhinia Variegata plant as potential lead candidates against SARS-CoV-2 transmission targets

Sunainy Ajrawat, Chhavi Thakur, Hemant Sood

European Journal of Molecular & Clinical Medicine, 2023, Volume 10, Issue 1, Pages 570-583

Bauhinia variegata, a medicinal plant commonly known as Kachnar, belongs to the Leguminosae family. It is primarily found in tropical and warm regions around the world. It is known for its high pharmacological and commercial significance due to the presence of terpenoids, flavanoids, tannins, steroids, reducing sugars and cardiac glycosides, which aid in the cure of number of diseases. This study carried out profiling of Phytochemicals of this medicinal plant by using several in silico computational methodologies against SARS-CoV-2 virus. The 3D structure of Phytochemicals and the three target sites of the SARS-CoV-2 were constructed using Swiss-Model server and pre-processed in BIOVIA Discovery Studio followed by molecular docking carried on with Autodock tools-1.5.6. The pharmacokinetic and toxicological properties of these compounds were assessed using the Swiss ADME and admetSAR web servers, respectively. The potential toxicities generated by these compounds, as well as the energies of molecule-target interactions, hydrogen bonding, and hydrophobic interactions, were determined and analyzed. According to the findings, Phytochemicals such as Beta-sitosterol, Lupeol, Ombuin, and Quercetin exhibited higher binding affinity than the two reference drugs Ivermectin and Lopinavir, taken as comparison in the study. According to the preliminary results, these bioactive compounds exhibit the most promising docking scores with the three target sites of Corona virus. Hence, they could be regarded as prospective  Phytochemicals found in Bauhinia variegata, potentially making this plant an active source of Phytochemicals required for the treatment of corona virus infections. Further experimental validation may necessitate the application of bioethics principles, laws, and regulations.

ANTICORROSIVE ACTIVITY OF PHOENIX PUSILLA LEAVES EXTRACT ON MILD STEEL IN HYDROCHLORIC ACID SOLUTION

A. Satish, S. Durgadevi and A. Leema Rose .

European Journal of Molecular & Clinical Medicine, 2023, Volume 10, Issue 1, Pages 1134-1140

Reducing metal corrosion has practical, financial, environmental, and aesthetic benefits. The usage of corrosion inhibitors is highly recommended. Since organic corrosion inhibitors leave behind dangerous chemicals in the environment, researchers have been seeking for non-toxic, biodegradable substitutes. Products made from plants have several advantages, including low cost, vast availability, and an endless supply of new products. They also have positive effects on the environment and are generally well-accepted by society. There has been investigation into the anticorrosive properties of phytochemicals in plants. Weight-loss methods and isotherm adsorption are used to examine the corrosion-inhibiting characteristics of Phoenix pusilla extract of leaves in 1M HCl solutions. The results showed that extracts have significantly reduced corrosion. The inhibitory effectiveness increased with increasing extract concentration. Adsorption isotherms and weight-loss study results support the existence of the adsorption mechanism (Temkin and Freundlich). The corrosion of steel is mimicked by using a plant extract in acidic circumstances. Results show that Phoenix pusilla leaf extracts have anti-corrosive capabilities and may improve surface protection by preventing active areas on metal.

GREEN CORROSION INHIBITOR AND ADSORPTION CHARACTERISTICS OF CARALLUMA INDICA STEM EXTRACT ON MILD STEEL IN ACID MEDIUM

A. Satish, S. Durgadevi and , A. Leema Rose .

European Journal of Molecular & Clinical Medicine, 2023, Volume 10, Issue 1, Pages 1141-1149

The stems of the plant Caralluma indica are used to create medication. Most people concur that one of the greatest ways to prevent metal from rusting is to use natural plant extract. They are easily accessible, non-toxic, friendly to the environment, biodegradable, extremely effective, and renewable resources. The effects of the Caralluma indica stem extract on the suppression of corrosion by 1M HCl solutions are the primary subject of this research. Weight loss procedures and isotherms adsorption are used to gather the necessary data. Based on the findings, it can be concluded that the extracts produced from 1M HCl solutions were effective corrosion inhibitors. When the concentration of the extract was raised, there was a corresponding rise in the efficiency of the inhibition. An inhibiting action can be produced by the Caralluma indica extract. The successful performance of the inhibitor can be attributed to phytochemical components with functional groups found in extracts of plants that have been adsorbed on the surface of the metal. Plant extract accurately mimics the corrosion of steel in acidic conditions, as indicated by data from weight deficit studies and adsorption isotherms, which validates the adsorption mechanism. Findings suggest that Caralluma indica stem extract acts as a corrosion inhibitor and can boost surface protection by covering up metal's active sites.

Phytochemicals And Antioxidant Of Methanol Extract Of Gracilaria Salicornia, Halimeda Gracilis, Halimeda Macroloba, And Hypnea Asperi From Tidung Island Coastal Region

Retno Widowati; Sri Handayani; Suprihatin .; Indra Lesmana Rahayu

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 1, Pages 896-907

The research aimed to find out the content of active compound of simplicia and the ability of methanol extract of Gracilaria salicornia, Halimeda gracilis, Halimeda macroloba, and Hypnea asperi as the antioxidant. Phytochemical testing of macro algae simplicia was conducted qualitatively to determine the presence of the active compound, i.e. phenolic, flavonoids, tanin, saponin, steroid, triterpenoid, alkaloid, and hidroquinon. The test method of antioxidant activity is the capture of free radical 1,1-Diphenyl-2-Picrylhidrazyl (DPPH). The active compounds which belongs to macro algae that are tested are flavonoids, steroids, and saponins. The results of examination of antioxidant activity showed the highest percentage of free radical capture of DPPH methanol extract of G. salicornia was 7,228%. The result of univariate ANOVA in antioxidant activity, indicating that there was an effect of concentration on free radical capture of DPPH (p ≤ 0,05) and did not show the interaction between species of macro algae and concentration. The highest percentage of interception DPPH free radical of methanol extracts of each macro algae tested i.e. G. salicornia, H. asperi, H. gracilis, and H. Macroloba respectively

ROLE OF MILLETS IN ENSURING FOOD AND NUTRITIONAL SECURITY

Pooja Kumari; Ashwani Kumar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 2544-2551

Millets are the small seeded grains of family Poaceae. They are able to grow on less fertile soils, have low water requirement and short maturity time. They are also nutrient dense in comparison to rice, wheat and maize. Their proteins are free from gluten and hence can be used an alternative to wheat flour for celiac patients. They are rich source of dietary fibre and phytochemicals like phytic acid and ferulic acid. Dietary fibre helps in the growth of probiotic microorganisms and hence millets can be used as a potential prebiotic source. Dietary fibre also reduces the glycaemic index of the food. Phytic acid helps in the reduction of cholesterol and also reduces the cancer. Ferulic acid is an antioxidant and exhibit strong free radical scavenging and anti-inflammatory activity. Nowadays, the millets have been use widely for the development of functional foods.

Phytochemical Screening And In Vitro Antioxidant Activity Of Enicostemma Hyssopifolium

K. P. Komal; V. M. BerlinGrace; D.David Wilson; Sardar Hussain

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 382-394

Medicinal plants and plant derived products have been part of the health care system since ancient human civilization. Traditional medicine uses plant extracts because of it’s antioxidant activity. In contrast usage of synthetic antioxidants are being restricted due to several limitations. Thus, there is always demand for newer and alternative phytomedicines. The aim of the present study is to analyse phytochemical components and to explore the In vitro antioxidant potential of methanolic extract of Enicostemma hyssopifolium (MEEh). The plant extract was prepared by organic solvents. The MEEh was used for GC/MS analysis and for exploring the prospects of Enicostemma hyssopifolium. The radical scavenging bioassays such as DPPH and ABTS were carried out to study in vitro antioxidant potency. The lipid peroxidation assay was performed to analyse their inhibitory effects and ferric reducing power of the extract was also evaluated. GC/MS analysis revealed that the plant is a rich source of phytochemicals such as 3,5-Dimethoxyacetophenone, Ergost-5-en-3-ol which are known to have antioxidant potency. In the antioxidant assay of MEEh the IC 50 was found to be 655.3>306.6 μg/ml. In lipid peroxidation assay, 33.45% of inhibition was found at 1000 μg/ml. The findings of the study revealed the potential of Enicostemma hyssopifolium as a source for natural antioxidants. It revealed that the plant could be a promising alternative agent in scavenging free radicals and may have ameliorative effect in complications associated with free radical damage.

Quality Evaluation Of Under-Utilized Crops Of Himachal Pradesh

A. Anand; Y.S. Dhaliwal; R. Verma; Manju .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4063-4072

Most of traditional crops have often been used for centuries, but have become increasingly neglected in times as more productive and profitable crops replaced them in farming systems. Almost about half of the humanity’s need regarding calories and proteins is met by three crops, wheat, maize, and rice, but there is an urgent need to promote crop diversification to avoid the vulnerable situations in future.. Under-utilized crops are often rich in nutrients when compared to more popular staple crops. They often contain high levels of protein, vitamins, phytochemicals and they can have a good macronutrient profile of fat, protein and carbohydrates. With regard to health superiority, national under-utilized crops for example pulses can be included as a promising food group. Thence, developing varietalhuman dietary sources e.g., amaranth, buckwheat, ricebean, adzuki bean, chenopodium and many more can play a important role to ensure nutritional security.

Antimicrobial Activity of Green Synthesized Silver Nanoparticles Room Sprayer from Arachis hypogaea Root Nodules

Govindaswamy Padmapriya; Alagesan Muthukumaravel; Kamalanathan Ashok; Janarthanan Senthil; Muthu Babu

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 4, Pages 2639-2646

Our research provides an update vision about the recent development in nanodrug by using eco-friendly and cost-effective silver nanoparticles from A. hypogaea root nodules for the first time. We also characterized these silver nanoparticles from various spectroscopic and microscopic methods. Their stability makes the present method a viable alternative to chemical and physical methods. Because of the least specificity of the reaction parameters, this process can be explored for large-scale synthesis of silver nanoparticles. Antimicrobial activity of silver nanoparticles room sprayer showed promising antimicrobial activity against S. aureus, E.coli and A. niger in the 100 ppm concentration and in future this kind of eco-friendly and cost effective silver nanoparticles room sprayer is needed to control many dread full diseases in the present scenario instead of using commercially available room sprayers. The investigation features a productive method to acquire bionanoaerosol that can be used against pathogenic microorganisms, thus contributing to solve this globally serious concern, especially given their being a limited choice of nanoantibiotic treatment.

Phytochemical analysis of α-amylase- inhibiting secondary metabolites of endophyte Penicillium brevicaule alba Thom

Gulyamova T.G.; Nasmetova S.M.; Ruzieva D.M.; Mukhammedov I.I,; Kadyrova G.Kh.; Karimova F. A.

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 829-836

The present work was aimed to identify phytochemicals in methanol extract P. brevicaule alba Thom - CC200, obtained from C. cristata , by qualitative and TLC analysis and evaluation of α-amylase inhibitory activity in vitro. It was determined the presence in the extract of glycosides, terpenoids, and saponins. The partitioning of the extract by TLC showed that inhibition of enzyme activity at 50,3% and 37,6% is associated with two compounds of saponin nature.

Phytochemicals as new class of antibiotics to control Multi Drug Resistance

Satish Kumar Sharma; Tejinder Kaur

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 6, Pages 1433-1442

Phytochemicals represent traditional knowledge of compounds effective in symptomatic relief and potential healing properties. The idea has emerged from the fact that natural resources such as neem, turmeric, cloves etc. have been used to cure health problems since a really long time. Even in the current modern world, people have more preference towards natural therapeutics than modern drug formulations. Antibiotics are the drugs which are most easily prescribed unnecessarily to attain extremely rapid symptomatic relief. The practice has led to natural selection of resistant bacterial strains which are spreading at an alarming rate. The world without antibiotics is horrible to contemplate and can cause uncontrollable mortality rate in future due to resistant bacterial infections. The review elaborates importance of phytochemicals as compounds of natural origin, easy production, industrialization scope, broad spectrum antibacterial capabilities to tackle the crisis of antibiotics in the ever growing era of multi drug resistance