Online ISSN: 2515-8260

Keywords : Malignancy


A HOSPITAL BASED OBSERVATIONAL STUDY TO CORRELATE THE CYTOLOGICAL FEATURES OF THE ASCITIC FLUID WITH CLINICAL DATA AT NEWLY ESTABLISHED TERTIARY CARE CENTER: A CLINICO-PATHOLOGICAL STUDY

Anil Kumar Sethiya, Madan Kumar Solanki, Hanuman Ram Choudhary

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 1696-1705

Background: The proper evaluation of ascitic fluid helps in narrowing the diagnostic dilemma faced by the physicians and helps in better management of the patients. Early and accurate diagnosis often depends on appropriate ascitic fluid analysis. The aim of this study to correlate the cytological features of the ascitic fluid with clinical data at newly established tertiary care center.
Materials & Methods: A prospective study was conducted in the Department of Pathology, in a tertiary health care centre over a period of one year. Cytological examination was performed to reveal important information regarding the causes of ascites and classified as benign (with or without infection/ spontaneous bacterial peritonitis), suspicious or malignant conditions. Cytomorphology of the cells was studied and documented paying attention to cellular arrangement, cytoplasmic features and nuclear characteristics. Clinical information of the patient and cytomorphological features of the smears were studied and the samples were categorized into benign (with or without infection/ SBP), suspicious of malignancy or malignant.
Results: Our study showed that the mean age was 55.67 years. Out of 30 cases, 14 were males and 16 were females, with male to female ratio being 7:8. Out of 30 samples, 26 (86.66%) were benign, 2 (6.66%) were suspicious of malignancy, and 2 (6.66%) were positive for malignancy. The most frequent clinical diagnosis was Alcoholic Liver disease comprising of 8 (26.66%) cases. The predominant cell type was Lymphocyte in 14 (46.66%) of cases, Neutrophil in 9 (30%) of cases, Reactive mesothelial cell in 5 (16.66%) of cases and atypical/malignant cells in 2 (6.66%) of cases. Histiocytic cells were also seen.
Conclusion: We concluded that non neoplastic and neoplastic conditions causing ascites can be diagnosed on ascitic fluid cytology. Increased neutrophil count is strongly suggestive of spontaneous bacterial peritonitis (SBP). The careful cytomorphological examination of ascitic fluid is a valuable, simple, rapid, inexpensive and reliable technique in the differential diagnosis of ascites, particularly in resource limited settings.

STUDY OF BIOPSY OF PROSTATIC LESIONS

Dr. Bairi Laxminarayana, Dr. V. Srinivas Kumar

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 2743-2755

Prostatic pathology is deceptively simple. Benign prostatic Hyperplasia, prostatitis and prostatic carcinoma are the three principal conditions involving the prostate accounting for more than 95% of lesions. Though the morphologic diagnosis of prostatic lesions, separating benign from malignant is relatively straight forward, there are several benign proliferations and normal histo-anatomic structures of prostate which mimic malignancy and their awareness is essential to avoid diagnostic pit falls.

Study of cell block technique in the cytodiagnosis of serous fluids at a tertiary hospital

Ashvini Pandit Mane, Hemant R. Kokandakar, A.R. Joshi

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 12216-12225

Background: Conventional smear is a less complicated procedure than that of cell block technique, it has lower sensitivity due of overcrowding of cells, cell loss, lack of architecture and also abundance of inflammatory cells and scarcity of representative cells contribute to appreciable difficulties in creating conclusive diagnosis on conventional smears. The aim of this study was to check the diagnostic yields of cell block technique in the cytodiagnosis of serous fluids.
Material and Methods: Present study was cross-sectional observational study, conducted in samples of ascitic fluid, pleural fluid, pericardiac fluid that were received in the cytology section, collected from patients clinically and radiologically diagnosed as having serous effusion. After reporting the conventional cytological smear the representative received samples were processed for cell block preparations.
Results: Findings of cellularity differs significantly, indicating better yield of cellularity in cell blocks as compared to conventional smear method. Distribution of findings of background obscured by blood or proteinaceous material differs significantly, indicating less degree of background obscured by blood or proteinaceous material on cell block preparation. Distribution of findings of morphology differs significantly, morphology is better appreciated with the help of cell blocks as compared to conventional smear method. Distribution of findings of architecture differs significantly, indicating better appreciation of architecture on cell blocks. Conclusion: Cell block method, concentrates cellular rich material, and it can increases cellular yield for diagnosis. Cell block increases cellular architecture pattern like acini, cells in sheets, papillae, which has helped to identify possible type or primary site of malignancy

KNOWLEDGE, ATTITUDE, PRACTICAL APPROACH OF DENTIST TOWARDS TREATING ORAL SUBMUCOUS FIBROSIS PATIENTS ATTENDING PRIVATE DENTAL HOSPITAL - A SURVEY BASED STUDY.

Nivethitha R; Dr.Deepika Rajendran; Dr. Saravana Dinesh S.P

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 1221-1236

Oral submucous fibrosis(OSMF) is a potentially malignant disorder of oral mucosa affecting mainly the population in south and southeast Asia. OSMF is precancerous condition caused by areca nut chewing characterised by restricted mouth opening, burning sensation and stiffness and blanching of oral mucosa.Complete regression of the condition had not been achieved in all cases with any of the present treatment regimen.The aim of the study was to assess the knowledge among dental students about the significance of Oral Submucous Fibrosis and it’s management. The questionnaire based study was conducted among 100 participants. A self administered questionnaire was used. The questionnaire based study was conducted through an online forum , google form software. The questions were formed to observe knowledge,attitude,and practical approach of dental students towards treating OSMF patients attending private dental hospitals .The data collected were stored and results were analysed by SPSS software. Out of 100 participants, 55% participants reported that females have an increased prevalence of OSMF compared to male. Majority of participants were aware that betel quid were the most common habits and blanching mucosa were the common features associated with OSMF. 60 % of participants were aware that stopping gutka / pan chewing habits alone will not resolve the problem in patients having trismus. Within the limits of the present study, students showed good knowledge on the various clinical and diagnosis aspects of Oral submucous fibrosis, however the knowledge on the management aspect of oral submucous fibrosis was moderate. A better knowledge of OSMF will endure safer health care services for the population.