Online ISSN: 2515-8260

Keywords : EBV

Immuno histochemical Detection Of The Expressed Protein Of P16 Gene In Colonic Adenocarcinomatous Tissues Infected With Epstein-Barr Virus

Basim M. Ibrahim; Saad Hasan Mohammed Ali; Jasim M. Karhoot; Khalil Ismail A. Mohammed

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 1252-1262

Background: Epstein-Barr virus (EBV) has been classified among group 1 listof infectious agentsthat is carcinogenic to humans. Some primary colonic cancers are often overexpressing the p16 protein while in contrast; others showed the high incidence of p16 repression by methylation. Objective: The present study was conducted to explore and compare the demo-pathological relation of EBV infection along with p16 expression in normal colonic mucosa, benign colon tumors, and colonic denocarcinoma. Patients and Methods: Seventy (70) colonic mucosal biopsies were enrolled and examined for EBERs and P16 gene expression which were done by chromogenic in situ hybridization (CISH) and immunohistochemical technique (IHC), respectively. These samples were belonged to (30) patients diagnosed with colorectal cancer mass, (25) from benign colon tumors (15) control tissues, which were proved by colonoscopy and histopathological examinations as apparently normal colorectal tissues. Results: Malignant colonic tumors showed positive results of EBERs -CISH detection in 56.7% (17 out of 30 tissues) while in benign colonic tumors were 24 % (6 out of 25 tissues), followed by the apparently healthy colonic control tissues were 20% (3 out of 15 tissues). The present positive p16-IHC results in malignant colonic tumors were 60% (18 out of 30 tissues) while in the benign colonic tumor and apparently healthy colon control tissues were 68% (17 out of 25 tissues) and 20% (3 out of 15 tissues), respectively. Conclusion: it was concluded from this study that the high rates ofEBV- EBERs expression could point to a their possible roles incolonic carcinogenesis, meanwhile, the p16- proteinexpression in colonic adenocarcinoma as compared to benign colon tumors and normal colonic mucosae could played roles in struggling against both EBV infection as well as colonic carcinogenesis.


Keerthana B; Gifrina Jayaraj; Gayathri R

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 1377-1383

Viruses cause familiar infections and diseases like common cold, flu. They invade living, normal cells and use those cells to multiply and produce colonies of cells.Viral infection is a proliferation of harmful viruses inside the body. Viruses infect the host body by introducing their genetic material into the cells. The main aim of this study is to explore detailed information about saliva and viral infections. This research is seen as a scoping literature review. In seeking to identify the relevant articles, we used common databases such as the Pubmed and Google scholar online websites. Totally 27 articles are found related to the topic. 12 articles are used in this study. From the articles collected and reviewed, it is understood that viral infections can transmit through the saliva and oral specimens, though saliva protects the body from infections. It is concluded that, as saliva has become the transmitter of the virus, we should maintain proper oral hygiene and oral health to prevent these viruses. Future studies have to be done on the viral infections causing cancer.