Online ISSN: 2515-8260

Keywords : Innovative


PRO-APOPTOTIC ROLE OF TECOMA STANS IN MELANOMA CELL LINE (A-375) - AN IN VITRO STUDY

Danda Nishitha; Lavanya Prathap; Dr. Selvaraj Jayaraman; Preetha. S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 617-627

Background :   Vigorous growth of cells leads to cancer. Tecoma Stans, a flower which consists of anti - cancer properties. One of such anti - cancer properties is the pro - apoptotic role in melanoma cell line (A-375). This is an in vitro study. The intervention is a hydroethanolic extract of Tecomastans on the melanoma cell line. The gene expressions chosen for this study are P53, caspase 3 and caspase 9. Aim of this study is to assess the pro apoptotic role of Tecomastans in melanoma.
 
Materials and methods : Human skin cancer (A - 375) was brought from NCCS, Pune, India. Cell viability test using MTT assay and Gene expression analysis for P53, caspase 3 and caspase 9 were carried out using MTT and PCR respectively. The results were analyzed using appropriate statistical tools using ANOVA and Duncan’s  test.
 
Results : The study suggestin MTT assay, the percentage of cell viability is observed to decrease on induction of Tecoma Stans.Caspase 9, mRNA  gene expression decreases on induction of dosages 300mg/ml and 400mg/ml of Tecomastans. P53 gene expression increases on induction of dosages 300mg/ml and 400umg/ml of Tecomastans. Caspase 3 gene expression doesn’t show any significance on induction of dosages 300mg/ml and 400mg/ml of Tecomastans.  
 Conclusion : Tecomastans consists of pro apoptotic property which is one of the anti cancer properties in melanoma when acted on the gene expressions P53, caspase 3 and caspase 9.

In Silico Analysis To Identify The Inhibitory Potential Of Endogenous Superoxide Dismutase On Apoptotic Markers Relevant To Breast Cancer.

Gopika. G.G; Lavanya Prathap; Selvaraj Jayaraman; Preetha. S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 638-648

Breast cancer arises when certain breast cells start to grow abnormally they  divide and replicate at a greater rate than normal cells. Regular exercise helps to reduce breast cancer by 10 to 20 percent. AIM: To analyse the inhibitory potential of endogenous over expression of superoxide dismutase (SOD) on apoptotic markers in breast cancer through silico analysis.
    The molecular docking analysis is a bioinformatic study. The endogenous substance SOD which is secreted after exercise is used as a target protein. The interaction of SOD with proteins relevant to breast cancer namely Bcl-2, Bcl-xl, Bax are included for docking analysis. The protein structure is retrieved using protein data bank, protein protein docking done using patch dock server followed by visualisation of protein -protein interaction using pymol.

TECOMA STANS HYDRO ETHANOLIC LEAF EXTRACT ANTIOXIDANT AND ANTIPROLIFERATIVE EFFECT ON LUNG CANCER CELL LINE A-549.

Faaizah Fathima; Lavanya Prathap; Dr. Selvaraj Jayaraman; Preetha. S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 716-728

Background: Tumor is a disease caused by abnormal cells dividing out of control. Lung tumor is a type of tumor that primarily affects smokers. Coughing, wheezing, chest pain, and other symptoms of lung tumor include these. Tecoma stans, popularly known as yellow bells, flowering perennial shrub endemic to the United States that belongs to the Bignoniaceae family of trumpet vines. It possesses antimicrobial, antispasmodic, antidiabetic, antibacterial, and trypsin inhibitor effects, among other things.
Aim: to investigate on the lung tumour cell line A549, Tecoma stans hydroethanolic leaf extract has an antioxidant and anti-proliferative effect.

Teaching Vocabulary And Grammar Through Task Based Learning Activities

Mr Anup Denzil Veigas; Dr David Wilson

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 201-211

Grammar and vocabulary are two vital components that exist in English language. A good command on both of these would relatively make the use of the language better and attractive on the part of the listeners. But today students find it quite challenging to have an excellent hold or command in these areas of grammar and vocabulary and students often end up on a poor scoring scale either in the English subject or in competitive exams or in their professional careers. In order to have an experience and learn the problems faced by students on these two challenging areas an experimental study was conducted among degree students among a semi- urban college and after analysing the results,task-based learning activities were implemented for learning. The outcome and analysis from the teaching learning experience makes the source of this paper. The rapid development and progress among the students become the result of innovative teaching practices over traditional teaching and it shows the essentiality for implementing innovative teaching practices among language teachers. The outcome of the study showed a significant improvement in the performance of the students and also their receptiveness to activity-based learning method over the traditional method that was highly admired. The outcome of the TBL method can be seen through the post test scoring that has relatively improved the overall performance of the learners.

ALGORITHM FOR TREATING PATIENTS WITH TRAUMATIC SUBDURAL HEMATOMAS

Mansur Agzamov; Firuz Normurodov; Akbar Tilyakov; Farhod Mavlyanov; Shukhrat Ziyadullaev

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 1749-1759

The study aimed to evaluate the effectiveness of the algorithm we developed when
choosing the surgical treatment tactics of acute subdural hematoma of the brain. A
retrospective analysis of 82 patients hospitalized from 2018 to 2019 was carried out in the
neurosurgery department of the Samarkand branch of the Republican Scientific Center for
Emergency Medicine with acute subdural hematomas. Group 1 included 53 observations,
where osteoplastic and resection craniotomy were performed for removal of subdural
hematomas. Group 2 included 29 observations, where subdural hematomas were removed
using a new minimally invasive method. Based on the data obtained, the algorithm developed
and implemented by us allowed us to provide emergency medical care most effectively and get
positive results. This algorithm can be used with all age groups of patients, regardless of the
severity of the injury. The algorithm for choosing the treatment tactics for acute subdural
brain hematoma is available on all stationary computers in all medical institutions and is easy
to use. In combination with the clinical and neurological picture, the results of radiation
diagnostics studies, the algorithm we developed is an innovative method of providing qualified
emergency medical care and surgical intervention that meets modern requirements.