Online ISSN: 2515-8260

Keywords : α-sma

Comparative evaluation of Immunohistochemical expression of MT-1 MMP, TIMP-1, TGF-β1, α-SMA in oral submucous fibrosis and oral submucous fibrosis with coexisting Oral Squamous cell carcinoma

Sharma Nidhi; Gawande Madhuri

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 1994-2002

Abstract: Background : „Oral submucous fibrosis” (OSF) is chronic, progressive, and
irreversible disease but also as oral potentially malignant disorder with high probability of
malignant transformation. From a clinical as well as the histological point of view it
appears to be of major importance to establish and understand the molecular nature of the
mechanism of malignant transformation in OSF .So that these molecules could be targeted
to formulate a standard treatment for high risk cases of OSF patients in need for better
prognosis. Material and Methods: 25 Cases of “oral submucous fibrosis .” and 25 Cases of
oral Submucous Fibrosis with coexisting oral squamous cell carcinoma will be analyzed.4
μm thick sections of formalin fixed paraffin embedded blocks were immunohistochemical
stained with Polyclonal rabbit MT-1 MMP antibody , Monoclonal mouse TIMP-1 antibody
,Monoclonal mouse TGF-β1antibody , Monoclonal mouse α-SMA antibody in each case
Statistical Analysis: Sample size formula based on difference between two proportions:
This study uses the following formula for the sample size n: n = (Zα/2+Zβ)2 * (p1(1-
p1)+p2(1-p2)) / (p1-p2)2,
Discussion: The Incidence of malignant potential in OSF has been estimated to be 7-13%².
After years of persistent efforts and research, satisfactory and definitive treatment modality
still eludes us. These markers could be used on regular basis to assess malignant potential
of OSMF. This will help to formulate and decide the standard treatment plan with a better
regime of target drug therapy


Andleeb Manhas; Amani Mahajan; Sadaf Antoo; Monika Negi; Muzaffar Parray; Swati Parhar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 1171-1183

Introduction: Odontogenic lesions are relatively common lesions and account for a substantial part of total oral biopsies by an oral pathologist. Some lesions like the keratocysticodontogenic tumor (KCOT) exhibit a locally aggressive behavior.Myofibroblasts (MFs) are specialized fibroblasts. These cells play a role in physiological processes like wound healing and pathologic conditions like reactive lesions, benign tumors, locally aggressive tumors and malignancies affecting oral cavity. Earlier, it was believed that the presence of Myofibroblasts (MFs) at the invasive front of the tumor was a part of the host defence mechanism against the tumor, but recent studies suggest that the presence of these cells at the tumor front promotes the growth and invasion of the tumor. The presence of Myofibroblasts has been reported in the stroma of many odontogenic lesions, including both cysts and tumors. Studies conducted both on odontogenic cysts and tumors revealed that the cells were particularly more in lesions with locally aggressive behavior like odontogenic keratocyst (OKC).
The aim of the study was to evaluate the role of Myofibroblasts in KeratocysticOdontogenic Tumor and its objectives were to compare the expression and distribution pattern of Myofibroblasts in KeratocysticOdontogenic Tumor (KCOT) as compared to their expression in Oral Squamous Cell Carcinoma and to derive a co-relation between the stromal Myofibroblasts and the known biologic behavior of KeratocysticOdontogenic Tumor (KCOT).