Online ISSN: 2515-8260

Keywords : Peri-implantitis


Assessment and comparison of Periostin levels in peri-implant sulcular fluid as a biomarker in healthy peri-implant sites and sites with periimplant mucositis and peri-implantitis

Dr. Anup U Shelke; Dr. Prasad V Dhadse

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2009-2016

Abstract: Background – Peri-implant diseases need early diagnosis and intervention in order
to prevent implant failure. Periostin is an enzyme imperative for periodontal health which
decreases with increasing severity of inflammation and levels revert back to normal after
treatment. Alterations in the levels of periostin with relation to peri-implant diseases is not yet
been investigated. This study aims to explore the role of periostin in peri-implant health and
disease. Objectives: To evaluate and compare the levels of Periostin in peri implant sulcular
fluid (PISF) in healthy peri-implant sites and sites with peri-implant mucositis and periimplantitis
with its clinical correlation. Method and design: Total of 66 implant sites loaded at
least one year earlier will be included in this study and divided into three groups. Group I
(healthy peri-implant tissues), group II (peri-implant mucositis), and group III (periimplantitis).
Levels of periostin in peri-implant sulcus fluid (PISF) acquired by microcapillary
pipettes acquired by micro-capillary pipettes will be determined from each group
using human periostin ELISA kit. Results: The results will be analyzed as levels of periostin in
peri-implant sulcular fluid recorded by ELISA in all the three groups and will be subjected to
intergroup comparison and will be presented after statistical analysis. Conclusion: Levels of
periostin in peri-implant sulcular fluid may prove to be a magnificent tool in early and prompt
diagnosis of peri-implant diseases which will help in planning and execution of appropriate
treatment, hence preventing further destruction of supporting tissues increasing longevity of
dental implants.

Determination Of Salivary Cytokines Level In Patients With Peri Implant Mucositis And Peri- Implantitis With Peri-Implant Maintenance Therapy

Dr. Ankita Priya; Dr. Shruti Singh; Dr. Rakhi kumari; Dr. Hiroj Bagde; Dr. Rupanjan Roy; Dr. Santosh Kumar Muradi

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 3692-3696

Background: The present study was conducted to determine the level of salivary cytokines in the absence or presence of periodontal and peri-implant maintenance therapy (TMPP).Materials & Methods: 48 patients with signs of peri implant mucositis and peri- implantitis were divided into 2 groups. Group I that performed TMPP regularly (GTP=24) and group II did not perform TMPP regularly, (GNTP=24). Cytokines such as IL-1β, IL-10, MMP-2, RANK, TGF-β and TNF-α were determined using diagnostic kit.
Results: There was non- significant difference in salivary biomarkers level between T1 and T2 in patients with peri implant mucositis and peri- implantitis (P> 0.05). There was non- significant difference (P> 0.05) in concentration of IL- I β, IL- 10, TGF- β, MMP- 2 and RANK between MP and PI patients in both GNTP and GTP group whereas TNF- a showed significant difference between MP and PI patients in GNTP group (P< 0.05).
Conclusion: The level of tumor necrotic factor alpha was higher in patients with peri-implantitis in patients with GNTP as compared to other cytokines.

Assessment of collagenase activity and MMP levels in GCF and in peri- implant sulcular fluid in healthy, chronic periodontitis and peri- implantitis patients

Dr. Ashima Trivedi; Dr. Shwetha N; Dr. Ankita Priya; Dr. Doddy Lokanathan Balaji; Dr. Anjaneya Mahapatra; Dr. Kaumudhi Kalla

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 5274-5278

Background: The present study was conducted to assess collagenase activity and MMP levels in GCF and in peri- implant sulcular fluid in healthy, chronic periodontitis and peri- implantitis patients.
Materials & Methods:40 patients of both genders were divided into 4 groups. Group I patients were healthy, group II had healthy dental implants, group III patients had periodontitis and group IV had peri-implantitis. Collagenase activity wasmeasured using a DNP-synthetic octapeptide.
Results: The mean gingival index was 0.6 in group I, 1.1 in group II, 2.2 in group III and 2.3 in group IV. Probing depth was 3.2 in group I, 2.5 in group II, 5.7 in group III and 5.1 in group IV. Collagenase activity per site was 1.7, 0.5, 15.2 and 14.7 in all groups respectively. Collagenase activity per ml was 9.2, 2.2, 16.4 and 21.6 in all groups respectively.
Conclusion: Peri-implantitis PISF contained higher collagenase-2 levels. GCF and PISF from severeCP and PI exhibited the highest activation of MMP-8 isoenzymes species.