Online ISSN: 2515-8260

Keywords : Penicillium brevicaule alba Thom- CC200


ISOLATION OF Α-AMYLASE INHIBITORS FROM METHANOL FRACTION OF THE ENDOPHYTIC FUNGUS PENICILLIUM BREVICAULE ALBA THOM

Saodat Nasmetova; Tashkhan Gulyamova; Dilorom Ruzieva; Iqbol Mukhammedov; Lilia Abdulmyanova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2174-2181

Abstract. Postprandial hyperglycemia is a significant risk factor involved in type 2
diabetes. The elevated level of postprandial hyperglycemia is caused by carbohydratehydrolyzing
enzymes, α-amylase, and α-glucosidase. Inhibition of these enzymes appears to
be an effective means for diabetes management. Recent studies suggested that endophytic
fungi of some medicinal plants produce α-glucosidase and α-amylase inhibitors. The
endophytic fungus P. brevicaule alba-CC 200, which exposes inhibition of α-amylase, was
isolated from the Celosia cristata plant. This article presents data on the purification of
inhibitory compounds from the methanolic extract of Penicillium brevicaule alba Thom-
CC200. Fraction of G-7 with the highest inhibitory activity of 76.5% isolated by adsorption
column chromatography. The yield of dry samples was more than 18 weight % (w/w) with
more than 60% purity. The HPLC and LC-MS of fraction G-7 indicated that the inhibitory
activity is these fractions associated with the triterpene saponins.