Online ISSN: 2515-8260

Keywords : carbohydrate energy exchange


MukaddaskhonKhamrakulova .; SabirovaGulchehra .; SadikovAskar .; SadikovKhumoyun .; NavruzovErnazar .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2810-2822

The energy supply of liver functions is carried out mainly by aerobic mitochondrial
processes. We have studied some aspects of carbohydrate energy metabolism in
mitochondria of the liver at the chronic influence of dichloroethane complex of factors,
noise and vibration on experimental animals’ organism. The research aims to study some
parameters of carbohydrate energy metabolism in mitochondria of the liver under the
chronic influence of dichloroethane, noise and vibration. The researchers carried out
experiments on 98 purebred white male rats weighing 160-180 g. Dichloroethane in the
form of 10% oily solution was injected into the animal’s stomach in a dose of 1/20 LD50
(255 mg/kg), in conditions of noise (95-110dBa) and vibration (2-16dBa) during 15 and 90
days. After the experiments end in 15, 30, 60 and 90 days after exposure cessation to
chemical and physical factors, the rats were decapitated and determined some indicators of
carbohydrate and energy metabolism in the liver and blood of experimental animals.
Presented results of experimental studies show that at repeated exposure to dichloroethane,
noise and vibration there was observed an increase in the intensity of anaerobic glycolysis
in blood, which is confirmed by the accumulation of lactic acid and reduction of glycogen.
Similar changes were observed in the reduction of activity of alkaline phosphatase in the
liver. Inhibition of enzyme activity (dehydrogenase) in all studied biospheres can be
explained by the influence of dichloroethane, noise and vibration on enzyme functional
activity. Inhibition of enzymes GDG, MDG, SDG and alkaline phosphatase in the liver is
observed at multiple effects of dichloroethane, noise and vibration. As a result of the
reduction of redox enzymes, under-oxidized products of anaerobic glycolysis of lactic,
pyruvic acid are accumulated and the level of glycogen is reduced. Prolonged exposure to
various chemical and physical factors in concentrations causing external effects in the
experimental animals’ organism is manifested by hidden changes in several physiological
reactions, biochemical indicators of the individual organs function and systems.


Khamrakulova Mukaddaskhon .; Sabirova Gulchehra .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2847-2862

Background:Experimental studies have confirmed the probability of
changes in some carbohydrate and protein metabolism indicators during
dichloromethane intoxication under the conditions of noise and vibration. It is
necessary to use Phytotea’ Gepalyuks’ with potassium for prevention and
treatment by correcting metabolic processes to avoid the adverse effects of
physical and chemical factors on the body.
Objective: Experimental study of the effectiveness of phytotea
“Gepalyuks” and potassium on carbohydrate energy metabolism of the liver and
blood under the influence of chemical and physical factors.
Methods: The experiments were conducted on 98 purebred white rats -
males weighing 160-180 years, contained in vivarium’s usual diet. Animals were
divided into control and experimental groups. Control animals received an
equivalent volume of drinking water, food and were kept in vivarium conditions.
Animals of the experimental group were divided into two groups: The first group
was intragastrically injected 10% of oiled dichloromethane solution in the dose of
1/20 LD50 (255 mg/kg) in conditions of noise (tape recording) at the level of 95-
105 dBA and vibration of 5-16 dBA during 90 days.
with intragastric administration of 10% oily solution of dichloromethane in a dose
of 1/20 LD50 (255 mg/kg), was administered a decoction of phytotea
“Gepalyuks”(1 ml of phytotea per 100 g of animal body weight) in conditions of
noise exposure (tape recording) at the level of 95-105 dBA and vibration of 5-16
dBA during 90 days. Phytotea “Gepalyuks” consists of immortelle sand flower,
yarrow herb, licorice root, peppermint leaves, corn snouts, and rosehip fruit.
Animals were slaughtered on 15, 30, 60 and 90 days from the beginning of the
experience. We took the liver, blood and determined biochemical indicators of
carbohydrate, protein, and pigment metabolism for research.
We studied some carbohydrate energy metabolism indicators to study the
effectiveness of phytotea“Gepalyuks” and potassium orotate under chronic
exposure to dichloromethane, noise and vibration.
Findings: When administered within 90 days, biologically active vegetable
and synthetic preparations in the form of decoction lead to an increase in the
activity of GDG, SDH and MDH and a decrease in the level of pyruvic and lactic
acids in serum and liver.
As a result of experimental studies, it was found that when administered
phytotea “Hepallux” in combination with potassium orotate for 90 days (15, 30,
60 and 90 days) under chronic exposure to dichloroethane, noise and vibration in
biochemical indicators - protein and lipid metabolism and activity of enzymes of
aliphatic amino acids remineralization (AST, ALT) and the content of cholesterol,
total and direct bilirubin in the liver and blood revealed a positive effect.
Conclusions: Application of phytotea “Hepallux” in complex
Potassium orotate causes a pronounced pharmacotherapeutic effect under
the toxic effects of chemical and physical factors - dichloroethane, noise and
vibration. Normalizes or approximates indices of carbohydrate energy (pyruvic,
lactic acids, the activity of GDG, SDG, MDG) metabolism and alkaline
phosphatase to those of the control group in blood and liver. The content of totalprotein, total and free bilirubins, the activity of AST, ALT in serum and liver is
approaching the norm.