Online ISSN: 2515-8260

Keywords : lead


EFFECTS OF LEAD ON THE DEVELOPMENT OF ZEBRAFISH EMBRYO

Tuan Van Ngo; Le Thi Lam .; Cang Ngoc Ly; Tram Thi Bich Tran; Huy Nghia Quang Hoang; Chi Nguyen Quynh Ho; Mai Thi Phuong Nguyen; Tram Le Ngoc Vo; Thuan Thi Tran; Nga Thi Nguyen; Long Thanh Le; Thao Thi Phuong Nguyen

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2681-2687

This study aimed to assess the effects of lead treatment on zebrafish embryo development. Embryos were treated with lead in various concentration at 0.1 μg/l, 1 μg/l, 10 μg/l, 20 μg/l, 100 μg/l. The results demonstrated that there was no difference in ratio of live embryo between control group and lead treatment group for 24 h. However, the live ratio of embryo was reduced in the high concentration of lead treatment from 72 h to 168 h. Lead treatment induced an increase heart rate in zebrafish embryos for 48 h and 72 h. In addition, the down-regulation of GADD45A (Growth arrest and DNA damage-inducible protein 45 alpha), GADD45G (growth arrest and DNA-damage-inducible 45 gamma), SOD1 (superoxide dismutase 1) and SOD2 (superoxide dismutase 2) was observed in zebrafish embryos for 168 h of lead treatment.

Correlation of peak amplitude ECG between leads Based on the condition of the heart

Sabar Setiawidayat

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 862-872

Non-invasive cardiac examination in standard clinic is still using 12-lead electrocardiograph. The results of the examination are presented on ECG paper or on the monitor screen. A normal electrocardiogram on one lead is not necessarily normal for the other lead, because each lead represents a certain part of the heart so that one by one is necessary. This examination takes time so that it can increase the stage of the disease if the patient turns out to be in an abnormal condition. This paper aims to correlate the peak amplitude of each lead to normal and abnormal heart conditions. If it is known that the peak amplitude is correlated between the leads, the other leads do not need to be checked, so that the diagnosis time will be obtained faster. Cardiac biosignal data that has been sampled with a frequency of 250 Hz is a discrete signal that can be stored digitally in a database. 10 samples of normal conditions and 10 samples of abnormal conditions were analyzed using Saphiro-Wilk so that the data were normally distributed. Spearman correlation analysis is used to get peak amplitude correlation between leads. The results showed that for abnormal conditions with a significance of 0.01 there was a correlation between the peak P lead I with leads III and V5, while for normal conditions there was a correlation between peak P lead I and leads V3 and V4. In abnormal conditions there is a correlation between peak R lead II and V6, while in normal conditions there is a correlation between peak R lead I and aVF.

POLLUTION EXPOSURE ON THE GINGIVAL LEAD LINE CASE ON STREET CHILDREN IN SDN KOTA LAMA 5 MALANG

Komang Fenny Gita Triesnanda; SatitiKuntari .; ArdiantiMaartrinaDewi .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 5, Pages 880-886

Background: Street children often carry out activities on the streets, which causes them to be exposed to lead at a much higher rate than the average person. Apart from being found in exhaust gases and motor vehicle batteries, lead can also be present in cigarette smoke. The high frequency of street children being exposed to different sources of lead will make them more at risk of developingthe gingival lead lines.Aim: To analyze the effect of pollution exposure on street children in SDN Kota Lama 5 Malang with the occurrence of gingival lead line. Method: This research is observational analytic research with a cross-sectional approach. The research samples are street children from SDN Kota Lama 5 Malang, with a total of 30 children. The data obtained were the results of direct measurements of the upper anterior gingiva (canines, central incisors, right and left lateral incisors).Results: A 2nd-degree description of gingival lead line is found to be the most prevalent, with a percentage of 53.3% of 30 street children having it.Furthermore, every street child who lives in the street for 7–12 months was found to have either a 2nd-degree or a 3rd-degree description of the gingival lead line. The longer the child is in the street, the higher the number of children affected by a 2nd-degree or a 3rd-degree description of the gingival lead line, followed by another risk factor such as their smoking habits.Conclusion: The pollution exposure on street children affects the incidence of gingival lead lines, such as the amount of time the children are in the street, the intensity of their eating habits while in the street, their smoking activities and their use of protective masks for themselves.

STUDY IN THE EXPERIMENT OF THE NATURE OF THE BIOLOGICAL RESPONSE OF THE ORGANISM AT SIMULTANEOUS AEROGENIC AND ORAL INTAKE OF LEAD AND DEVELOPMENT OF ITS PERMISSIBLE VALUES IN THE AIR, WATER AND FOOD PRODUCTS

Mirzakarimova Malohat .; Kamiljanov Abduvakhit .; Adilov Utkir .; Dinora Khashirbaeva .; Sagdullaeva Barno .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2823-2835

Lead and its compounds are continuously detected in environmental objects at
various concentrations and doses. Lead is a general plasma poison that affects the nervous,
bone, endocrine system, blood and blood vessels, parenchymatous organs, gastrointestinal
tract, metabolism, reproductive function, immune status and resistance. In this regard, there
is an acute need to study in the experiment features of the response of the body of rats at
the same time aerogenic and oral intake of lead and the development of its permissible
values in the air, water and food products, taking into account the complex effects.
Objective: to determine experimentally the peculiarities of biological response of
experimental animals at simultaneous aerogenic complex intake of lead and development
of its permissible values in air, water and food products.
Methods: Concentration (dose) - time-effect relationship was used. For inhalation,
200-liter chambers were used in the experiment, proposed by B.A. Kurlyandsky. Modern
highly sensitive biochemical, physiological, gonad and embryotoxic indicators were used
to assess the resorptive toxic effect of lead.