Online ISSN: 2515-8260

Keywords : Fluorescein

Lab-Build Very Simplefluorimeterflow injectionsystem with Assistanceof 3D printer

Ali S. Namea; K.H.Al- Sowdani

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 3818-3827

This study focusing on build-up and designing a fluorimeter flow injection system with assistance of a 3D printer..
The fluorimeter as new detector in our laboratory is a very simple fluorimeter was designed and built-up by the aid
of a 3D printer to housing in which all the components are supplied from the local market. It consists of three major
parts. Firstly, a simple, low-voltages, and long-lived light-emitting diodes (LED) with 0.25 watt. The second parts is
450 μL flow cell QS Hellman with four faces in order to select precisely the 90 ₒ angle between the source and
third components, the LED arrays detector . All these components were put in small plastic box which the internal
and external covers were made from black plastic which thought to prevents the unwanted light from reaching the
detector. The device is very light weight (165.5 g ) and the fluorimeter box dimensions were 11cm in length , 8cm in
width and 3cm in height.Two Microcontrollers Mega and UNO types were used as data a data-logger and controlled
the home-made mini peristaltic pump, respectively. A direct fluorimetric determination of fluorescein dye as an
example which was carried out in this work. This thought will be made the construction system a very simple
fluorimeter since the dye excited and fluorescent in the visible range.Linearity was found in the range ( 0.2-1.6 x 10-
4mol/L) with a regression coefficient of eight points was 0.9989, r.s.d% for ten replicates of was 0.84% and detection
limit was 0.1×10-4mol/L. The sample throughput was 200 samples per hour. The dispersion coefficient of the
manifold was 1.04. The lab-build semi-automated fluorometeric FI system was reliable, fast and low cost which is
matching our undergraduate laboratories with low budget.