Online ISSN: 2515-8260

Keywords : quercetin


Role of Polyphenolic Compounds in Management of Oxidative Stress Associated With Glaucoma

Chandrashekhar Mahadeo Chakole; Meenakshi Kanwar Chauhan

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 2064-2084

Glaucoma is a first rank common cause of irreversible vision loss. It is also
recognized as a neurodegenerative disease which progress with age, results in optic
neuropathy. The exact cause of glaucoma remains unclear although oxidative stress
considered as one of the reasons for cell death in the retinal ganglion cell and retinal
pigment epithelium. Oxidative stress could result after imbalance between formation and
utilization of reactive oxygen species. Current pharmacotherapy of glaucoma includes
lowering down of elevated level of IOP, which is not sufficient enough to retard
irreversible vision loss in some instances. Hence, alternative neuroprotective therapy is
warranted. Polyphenolic compounds possess antioxidant, anti-inflammatory properties and
also show the neuroprotective effect in an experimental model. Amongst the natural
polyphenolic compounds resveratrol, curcumin, rutin, quercetin, myricetin have been
studied and showed potential as neuroprotection against cell apoptosis. Moreover, the extra
supplement of a polyphenolic compound may also improve antioxidant status, which was
underestimated in glaucoma disorder. Despite the potential, the polyphenolic compounds
yet to explore for clinical use in ocular disorder. Hence it is an excellent opportunity for
the future researcher to transform these substances from lab to clinic as neuroprotectants
in glaucoma.

In-Vitro Anti-Proliferative Effect Of Flavonoid Extract Of Amaranthus Viridis (Kolitis) Leaves Against MCF-7 Breast Adenocarcinoma Cell Line

Allysa Camille M. Malicdem; Michkaella Lorena O. Aducal; Jireh C. Cuasay; Denise Ansherina A. Dalisay; Alexander I. Mendoza; Glenda Marie E. Napiza; Carina R. Magbojos; Oliver Shane R. Dumaoal

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4129-4140

Cancer is one of the major concerns in health and medicine. Studies have been done by previous researches on the medicinal value of flavonoids present in Amaranthus viridis. However, limited claims were available in terms of their anti-proliferative ability against cancer cells. Flavonoid extracts were prepared by semi-purification using ethanol and ethyl acetate. Phytochemical screening using UV-Vis spectrophotometry was used to verify the presence of flavonoids. MTT cytotoxicity assay using MCF-7 breast cancer cells was employed to determine the cytotoxic effect of the plant extract. Four concentrations of the extract of A. viridis leaves were used as treatment to cultured cells. Doxorubicin and DMSO were used as the positive and negative control, respectively. An inhibition concentration (IC50) of 18.33 ug/mL of the extract was reported after the assay, indicating that the administration of the treatment caused the death of the cells as seen when the yellow dye was not reduced into a purple formazan. Statistical analysis on the IC50 of doxorubicin and the A. viridis extract showed significant relationship, which indicated that doxorubicin has a more potent cytotoxic activity, though the extract had promising effects as seen in its IC50 being less than 30 ug/mL. These results can be attributed to the presence of the flavonoid quercetin which has been found out to possess cytotoxic effects against cancer cells. With these findings, a more natural, less toxic, cost-efficient and more readily available treatment regimen can be utilized for the management of cancer.