Online ISSN: 2515-8260

Keywords : Orange

Potential Synergistic Antimicrobial Activity of Extract from Peelings of Common Local Fruits: An In-Vitro Analysis

Laarni M. Villanueva- Magsombol; Carina R. Magbojos- Magtibay

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4175-4185

The objective of this study is to investigate the potential synergistic antimicrobial activity of methanolic extract from peelings of Philippines’ common local fruits, namely, orange, mango, and papaya. Peelings from these local fruits were properly washed, dried and powdered. Extracts from the peelings were obtained using methanol as the solvent, concentrated in a rotary evaporator set and were finally reconstituted in 20% dimethylsulphoxide (DMSO).The preliminary determination of antimicrobial effect of the individual fruit peelings was tested using plate count method. A two-fold dilution of the individual formulated extracts were done and combined, after which the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Staphylococcus aureus and Escherichia coli isolates were determined. MIC results of the mango extract showed best antimicrobial effect, which worked at a minimum concentration of 6.25mg/ml on both isolates. This was confirmed by the MBC, which showed the absence of growth of viable microorganisms after 24-hour incubation on nutrient agar. Orange and papaya were found effective against S. aureus at a minimum concentration of 50mg/mL. However, these extracts did not demonstrate individual antibacterial activity against E. coli. The combination of mango and papaya was the most effective among the three sets of extracts used in the synergistic antibacterial activity. It was found to be effective against E. coli at a minimum concentration of 6.25mg/mL and against S. aureus at 25-100mg/mL. Other combination of extracts such as orange-mango and orange-papaya exhibited synergistic antibacterial activity at higher concentrations. Moreover, orange and mango combination had a better synergistic activity against E. coli than S.aureus while orange and papaya if combined has greater synergistic activity against S. aureus than E. coli. From this study, it can be concluded that the combination of the extracts of mango and papaya has a more potential synergistic antimicrobial activity against S. aureus and E. coli.

Estimation Of Phenolic Compounds And Evaluation Of Their Antioxidant Activity Of Some Parts Of The Orange Plant (Citrus Sinensis L.)

Iman Mohsin Kadhom; Nagham Saddon Ibrahim; Najim Abdullah Al-zubaidy

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 4811-4822

Orange fruits were collected from one of the gardens of Baqubah / Diyala / Iraq in January of 2020, while samples of leaves and flowers were collected in March of the same year, for the purpose of completing the research procedures. The present study aims to estimation of phenolic compounds and evaluation of their antioxidant activity of the extract of some parts of the orange plant. The results show a variation in the parts of the orange plant in their content of phenolic compounds. Moreover, the statistical analysis pointed to the presence of significant differences at the probability level of P <0.05 among the parts of the orange plant, in Total Phenolic Content (TPC) and Total Flavonoid Content (TFC). The leaves are superior in their (TPC) which record the highest in comparison with the juice, which recorded the 1-concentration of 145.71 mg( GAE) .g. Though the highest (TFC) in the mesocarp of fruit is 1-lowest (TPC) 57.18 mg( GAE) .gin the juice, which gave the 1-compared to 5.81 mg.Querecetin. g 1-mg Querecetin .g 11.14lowest (TFC).
On the other hand, the result of statistical analysis showed significant difference among the parts of orange plant at the probability of P <0.05 in antioxidant activity. The result showed that the highest antioxidant activity, detected by using the DPPH scavenging . While the lowest value of the free radicals DPPH 1-test of the flowers is 2.64 mg. mLwas obtained from exocarp. The statistical analysis 1-inhibition, which was 0.86 mg. mLresults indicated a positive correlation among the (TPC), (TFC) and the antioxidant activity of DPPH free radical test with value of 0.385 and 0.484 respectively.