Keywords : methylation specific PCR
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 5, Pages 55-61
Breast carcinoma is the most commonly diagnosed cancer and the leading cause of cancer death. Breast cancer also produces and is influenced by ectopic hormones. Beta Human Chorionic Gonadotropin (hCG) is one such hormone and is encoded by chorionic gonadotropin beta (CGB) genes. The aim of this study was to determine the CGB gene methylation in breast cancer tissues and compare them with normal tissues.
Materials and methods: After approval from Institutional Ethical Committee (IEC), consent from patients were obtained. Normal and tumour tissues from breast cancer patients were taken. DNA was isolated from normal and tumour tissues. Post bisulfate conversion samples were processed for qPCR using methylation specific primers for the set of selected CGB genes and SYBR green.
Results: 1-2M was found to be significantly higher among the normal tissues (50.22). 3-9M was found to be 65.93 in tumour tissues and 5.05 in normal tissues and this was significant.
Conclusion: 3-9 M is significantly higher in tumour tissues compared to normal tissues and 1-2 M is significantly higher in normal tissues. This suggests that there are 2 different types of beta hCG secreted by two different types of genes and this can be used for further analysis as a part of future projects. This may help in formulating a new treatment process and may also be used as a tumour marker in high risk patients