Online ISSN: 2515-8260

Keywords : Renal failure


The Oxidative Effect Of Nlrp3 And Cyp2e1 In Development Of Renal Failure Associated With Hypertension And Diabetes Mellitus Diseases

Alyaa Abdulhadi Salih; Kareem Hamadx Kareem Hamad Ghali2

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 636-644

Renal Failure (RF) is devastating pathology with several causative factors, resulting in dialysis or kidney transplantation needed by end-stage renal disease (ESRD. The progression of RF is closely related to systemic oxidative stress, which causes various complications such as hypertension and diabetic mellitus . The current study was focusing on the oxidative effects of NLRP3 and CYP2E1 gene expression in the pathological development of renal failure disease with hypertension and diabetic mellitus as complications associated diseases. This study involved 100 patients diagnosed clinically and seriologicaly with renal failure 52 males and 48 females, their age from 17 to 80 years . Also the study comprise (18 males and 12 females) healthy volunteers as control group. RNA extracted from blood samples and converted to cDNA which subjected to real-time PCR for detection gene expression . The results detected that Hypertension and diabetic mellitus are the important risk factors that causes and advances renal failure disease . NLRP3 and CYP2E1 have significantly overexpression (1.60 folds and 5.37 folds ) respectively in RF patients compared with healthy group ( p <0.05). However, the expression of NLRP3 and CYP2E1 increase in diabetic mellitus (2.16 and 1.58 ) respectively than hypertension in RF patients with and control group (p<0.05). Diabetic mellitus generate free radical consequently causes ROS, hence elevated expression in oxidative NLRP3 and CYP2E1 genes in diabetic RF patients .

Electrolytes System Disorder And Liver Enzyme Alteration In Adult Men With Hepatocellular Injury

Alzahraa Ibrahim Abdulmajeed; Wafaa Sh. Al – Zuhairi; Ekhlas Abdallah Hassan

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 6, Pages 805-812

Objective: The current study aimed to estimate the magnitude of liver hepatocellular damage in adult men suffering from advanced stages of liver infection considering various important variables (age, BMI, TSB, DSB, GOT, GPT, Na+, K+, Cl-). Background: The main cause of hepatocellular injury is viral hepatitis C. Development of this disease leads to transformation of normal liver tissue into fibroses and nodules (cirrhosis), and it is associated with numerous complications, including gastrointestinal bleeding, ascites, renal function disorder and electrolyte disturbance. Method: The sera of all participants, including 25 patients with VHC and 15 cirrhotic patients, were collected for comparison with 23 control donors (healthy men). The present study was conducted in Baaqubah General Hospital/Diyala. Serum electrolyte concentrations were measured by spectrophotometry assay. Results: significantly decreasing sodium concentration in cirrhotic patients and statistically increasing chloride concentration in patients with liver disease compared with the control (P value < 0. 05). Furthermore, a number of disorders that effect of the liver enzymes levels, Conclusion: The essential decrease in sodium (Na+) concentration was observed in cirrhotic men caused by reduction in renal perfusion. This result is in contrast to that of serum chloride, which increased in patients with liver diseases.