Online ISSN: 2515-8260

Keywords : superoxide dismutase


EFFECTS OF LEAD ON THE DEVELOPMENT OF ZEBRAFISH EMBRYO

Tuan Van Ngo; Le Thi Lam .; Cang Ngoc Ly; Tram Thi Bich Tran; Huy Nghia Quang Hoang; Chi Nguyen Quynh Ho; Mai Thi Phuong Nguyen; Tram Le Ngoc Vo; Thuan Thi Tran; Nga Thi Nguyen; Long Thanh Le; Thao Thi Phuong Nguyen

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2681-2687

This study aimed to assess the effects of lead treatment on zebrafish embryo development. Embryos were treated with lead in various concentration at 0.1 μg/l, 1 μg/l, 10 μg/l, 20 μg/l, 100 μg/l. The results demonstrated that there was no difference in ratio of live embryo between control group and lead treatment group for 24 h. However, the live ratio of embryo was reduced in the high concentration of lead treatment from 72 h to 168 h. Lead treatment induced an increase heart rate in zebrafish embryos for 48 h and 72 h. In addition, the down-regulation of GADD45A (Growth arrest and DNA damage-inducible protein 45 alpha), GADD45G (growth arrest and DNA-damage-inducible 45 gamma), SOD1 (superoxide dismutase 1) and SOD2 (superoxide dismutase 2) was observed in zebrafish embryos for 168 h of lead treatment.

Study Of Genetic Polymorphism In Oxidative Stress Related Genes And Their Association With Gastrointestinal Cancer: A Case Control Study From Rural Population Of South Western Maharashtra

Madhavi N. Patil; Anand Gudur; Rashmi Gudur; Satish Kakade; Sandeep Kadam; Suraj Pawar; Kailas D. Datkhile

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 42-52

Oxidative stress is known to be one of the major factors involved in the development and progression of cancer. Superoxide dismutase and Catalase plays an important role in the primary defence against oxidative stress. The present study was therefore undertaken to investigate the association between polymorphism of superoxide dismutase (SOD1, SOD2, SOD3) and Catalase (CAT1 & CAT2) and the gastro intestinal cancer risk in the rural population of the south western Maharashtra. The present case-control study included 200 confirmed gastrointestinal (GI) cancer patients and 400 age and gender matched healthy controls. The polymorphism in the Superoxide Dismutase and Catalase genes was studied out by PCR-RFLP method. When we studied the genotypic frequency of SOD1, SOD2, & SOD3, SOD2 (rs1141718) showed the negative association with the GI cancer risk [OR: 0.3097, CI: 0.1727-0.5553, p <0.0001]. There was no significant association found between SOD1 & SOD3 of superoxide dismutase and CAT1 & CAT2 of Catalase gene polymorphism and susceptibility of GI cancer. The present study shows no significant association of SOD1 (G allele of rs 2070424), SOD3 (A allele of rs2536512), CAT1 (T allele of rs7943316) and CAT2 (rs1001179) with the development of gastrointestinal cancer in the rural population of south western Maharashtra from India. However, SOD2 (rs1141718) shows the negative association with the development of GI cancer.