European Journal of Molecular & Clinical Medicine

 
BACKGROUND: Tumor is an abnormal cell growth that spreads to other organs. Breast tumor develops in the tissues of the breastRegular exercise can help to lower the chance of developing breast tumor. For the year 2020, the expected incidence of tumor patients in India was 679,421 (94.1 per 100,000) for males and 712,758 (103.6 per 100,000) for females.. AIM: To analyse the endogenous over the expression of catalase as an inhibitor of EMT signaling in breast tumor through molecular docking.
 
MATERIALS AND METHODS:
            The molecular docking analysis is a bio informatic study conducted in a private dental college. The endogenous substance catalase which is secreted after post exercise is used as our target protein. The interaction of catalase with the proteins relevant to breast tumor namely Vimentin, Beta-catenin, Ecadherin are included for docking analysis. The protein structure is retrieved using protein data bank, Protein protein docking done using patch Dock server followed by visualisation of protein-protein interaction using pymol.
 
RESULT:
             The surface representation of catalase with Vimentin, Beta-catenin and Ecadherin showed good shape complementarity.The results showed that Catalase forms strong interaction with Vimetine, Beta catenin, Ecatherine proteins in terms of hydrogen bond interaction, hydrophobic and non bonded interaction. Through this interaction these proteins might control the overexpression of catalase activity in breast tumor.
 
  CONCLUSION:
           From the obtained result it can be concluded that catalase may have a protective role against breast tumor through its interaction with Vimentin, E-Cadherin, Beta-Catenin. The present study has suggested a possible mechanism of catalase in the inhibitor of EMT signaling in breast tumor.

Oxidative stress is known to be one of the major factors involved in the development and progression of cancer. Superoxide dismutase and Catalase plays an important role in the primary defence against oxidative stress. The present study was therefore undertaken to investigate the association between polymorphism of superoxide dismutase (SOD1, SOD2, SOD3) and Catalase (CAT1 & CAT2) and the gastro intestinal cancer risk in the rural population of the south western Maharashtra. The present case-control study included 200 confirmed gastrointestinal (GI) cancer patients and 400 age and gender matched healthy controls. The polymorphism in the Superoxide Dismutase and Catalase genes was studied out by PCR-RFLP method. When we studied the genotypic frequency of SOD1, SOD2, & SOD3, SOD2 (rs1141718) showed the negative association with the GI cancer risk [OR: 0.3097, CI: 0.1727-0.5553, p <0.0001]. There was no significant association found between SOD1 & SOD3 of superoxide dismutase and CAT1 & CAT2 of Catalase gene polymorphism and susceptibility of GI cancer. The present study shows no significant association of SOD1 (G allele of rs 2070424), SOD3 (A allele of rs2536512), CAT1 (T allele of rs7943316) and CAT2 (rs1001179) with the development of gastrointestinal cancer in the rural population of south western Maharashtra from India. However, SOD2 (rs1141718) shows the negative association with the development of GI cancer.

As a result of research, it has been established that synthetic karate pyrethroid causes functional changes in antioxidant enzymes in microsomes and mitochondria. An important aspect of our study was the comparison of the taxation rate when animals were poisoned with a karate pesticide and when animals were protected from poisoning with an antioxidant of plant origin. The results obtained indicate that the plant antioxidant factor (PAF) has protective properties and accelerates the restoration of enzyme activity.