Online ISSN: 2515-8260

Keywords : Anticancer


Evaluation Of Anticancer Effect Of Ethanolic Extract Of Lepidagathis Pungens Nees., Whole Plant By Mtt Assay- An In Vitro Study

Manoharan Dhanalakshmi; Subramaniam AnandaThangadurai

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 1027-1038

Extracts from natural products, especially plants, have served as a valuable source of diverse molecules in many drug discovery efforts and led to the discovery of several important drugs. Lepidagathis pungens Nees., (L.pungens) is a herb of tropical Asia belonging to the family Acanthaceae. The present study deals with the in vitro anticancer activity of ethanolic extract of L.pungens against L6, Ehrlich Ascites Carcinoma (EAC), human breast cancer cells ( MCF 7), human cervical cells (He La) and human hepatocellular carcinoma cell lines (Hep G2). MTT assay was used to assess the in vitro anticancer activity. The extract at various doses were treated against all the cell lines. The parameters analyzed were percentage of cytotoxicity, percentage of cell viability and IC50 values. In results the ethanolic extract of L.pungens showed more potent activity against MCF 7 and EAC cell lines but moderate activity against He La and Hep G2 cell lines. The anticancer nature of the ethanolic extract is due to the presence of phytoconstituents like flavanoids, alkaloids, phenolic compounds, terpenoids, etc. However, further indepth studies are required before final conclusion on the mechanisms involved could be drawn to explain the observed activity. In conclusion, the ethanolic extract of L.pungens possess anticancer activity against various types of cancer cells, correlated to their total phenolic content.

In-Vitro Antioxidant And Anticancer Evaluation Of Newly Synthesized N-(5-Oxo-2-Phenylimidazolidin-1-Yl)-4-(3,4-Dihydroxyphenyl)-6-Methyl-2-Oxo-1,2,3,4-Tetrahydropyrimidine-5-Carboxamide

S. Shanmugam; K. Neelakandan; M. Gopalakrishnan; S. Pazhamalai

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 353-370

A novel N-(5-oxo-2-phenylimidazolidin-1-yl)-4-(3,4-dihydroxyphenyl)-6-methyl-2-oxo-1,2,3,4-tetrahydropyrimidine-5-carboxamide (1) was synthesized using Biginelli reaction at room temperature. The dual heterocyclic compound 1 was characterized using the analytical techniques such as FT-IR, 1H & 13C-NMR, Mass analysis, UV-visible and fluorescent spectroscopic system. The data on charge density was used to explain the characteristics of molecular systems. In addition, in the form of the complete and partial density of states, the HOMO-LUMO energy gap, electrostatic potential map and some quantum chemical insights have been obtained. Furthermore, to demonstrate the possible applications of dihydropyrimidinone 1 in nonlinear optics the polarizability and first hyperpolarizability were measured. Molecular docking is also determined in order to illustrate the overexpression of estrogen receptor in 80 % of 2J9M protein. The compound 1 was subjected for its antioxidant (ABTS) and anticancer activity (DPPH) by in-vitro analyses and found with substantial antioxidant activity.

In Vivo Acute Toxicity, Anticancer And Antioxidant Assay Evaluation Of Thunbergia Grandiflora.

Mahesh Babanrao Thorat; Dr. Neetesh Kumar Jain

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3483-3498

Thunbergia grandiflora, commonly called blue trumpet vine, sky vine or sky flower belongs to Acanthaceae, which is native in Australia, Central America, the West Indies, Africa, and numerous islands in the Pacific including Hawaii, Fiji, French Polynesia, Palau, and Samoa. In the present investigation, Thunbergia Grandiflora extracts were studied for in-vivo acute toxicity, anticancer and antioxidant activity. According to the toxicity study, 200 and 400mg/kg body weight was selected as dose. In the evaluation of anticancer activity the tumour yield and tumour burden were not detected up to 4th weeks; but in 8th week, observed the formation of tumours and it become maximum in 16th week in all treated groups of mice. In carcinogenic control group of mice maximum tumors activity was observed due to carcinogenic nature but it got decreased after the treatment with sample 1,2,3 and isolated compound. Also, carcinogen increased LPO and reduced SOD, GSH, and catalase in lung homogenate. Extracts significantly reversed the effect of carcinogen on LPO level. The isolated compound was found more effective than the extracts (200 mg/kg an 400 mg/kg) on LPO, SOD, GSH and catalase. Moreover the extract of Thunbergia grandiflora showed down regulated effect on the levels of TNF-α and IL-6.