Keywords : Bacterial
ISOLATION AND IDENTIFICATION of SOME BACTERIAL PATHOGENS FROM SUBCLINICAL MASTITIS of SHEEP in KIRKUK CITY, IRAQ
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 9, Pages 383-391
Background:- Mastitis is the term for a bacterial infection of the udder. It is most
common in ewes raising multiple lambs or with high milk production. Most cases occur
during the first weeks after lambing or immediately before weaning. Good ewe
nutrition and providing a clean lambing environment are important factors in reducing
the incidence of mastitis.
Objective: The study was focused on using of rapid & efficient methods for detection of
Subclinical Mastitis in 4 small holder dairy flock in central of Kirkuk &its affiliated
Material and Methods:- A total of 94 milk samples sheep were collected from four
farms in October to December 2018 to determine pathogens responsible for subclinical
mastitis in sheep.
Results:- Screening Subclinical Mastitis(of SCM) were done using California mastitis
and White Slide Test (WST). the prevalence percentage of SCM in California mastitis
and White Slide Test according the early stage lactation was(74 % and 70%)
respectively. While the prevalence percentage of SCM in California mastitis and White
Slide test according the according the dry period lactation was(66 % and 64 % )
respectively. identification of the isolates was achieved using Gram’s staining,
hemolytic pattern, colony morphology, Catalase , Coagulase test, IMVIC test and
confirmation of bacteria species by using Vitek 2 compact after identification to the
primary biochemical tests using Analytical Profile Index. Bacteriological examination of
all milk samples found the presence of (82.85% ) isolates where Staphylococcus was
predominant species (74.28 %) and the coagulase negative Staphylococcus (25.7% )
species was identified at the least bacteria .than staph. aureus, while environmental
pathogen represented E. coli second common pathogen followed by klebsiella
pneumonia (14.8% ) , Manhemia haemolytica (12.8 %) proteus spp.( 11.4%) And low
percentage to the Enterobacter spp. (4.3 %) .
Conclusion:- subclinical mastitis seems to be, as deduced from the high prevalence
observed in this study, an important health problem for milking sheeps in the Kirkuk
Application of VITEK system could be practiced at selected samples from time to time
to confirm identification of causative organisms.