Online ISSN: 2515-8260

Keywords : pathogens


Describing the Major Pathogens Causing UTI Among Patients, The Prevalence Of UTI and Establish Susceptibility Pattern of Antimicrobial Resistance

Dr. Aarti Akhand, Dr. Ramanath Karicheri

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 4909-4913

Background & Method: The Study was carried out among UTI patients in Index Medical College and Associated Hospital at Khudel Village in Indore that gives healthcare to surrounding area and is used as teaching and referral hospital. The study targeted both male and female outpatients and inpatients presenting with symptoms and signs of UTI which include dysuria, polyuria, fever, nausea, and flank pain were sampled for this study. Baseline demographic data including age, sex, level of education and risk factors such as catheterization, history of UTI, also out and in patients were also collected.
Result: The 385 urine specimens processed, 112(29.0 %) showed significant growth whereas the majority of the urine samples that is 273 (71 %) showed no growth. Fresh samples were collected from patients by mid-stream catch method in sterile universal bottles (Plate 1). The assessment of associated risk factors showed gender (χ2 = 0.116, P=0.0412), age group (P=0.0120), History of UTI (χ2 =0.555, P=0.004) and symptoms of UTI (χ2 =0.895, P=0.017) were significant. Level of education (χ2 =2.742, P=0.523) and catheterization (χ2 =0.17, P=0.054) were not significant.
Conclusion: The prevalence of UTI in Index Hospital was 29.1 %. E. coli was the highest among 120 the isolates. Females had a higher (62.1 %) prevalence of UTI than males (37.9 %). Urinary tract infection was associated with the previous infection and with patients who had the history of catheterization. All isolates were sensitive to nitrofuratoin, cefotaxime and amoxicillinclavulinic acid. High resistance was observed in gentamicin, nalidixic acid, ampicillin and cotrimoxazole. In the 120 isolates were resistant for more than two antibiotics were recorded in 108 (90 %) isolates. Antibiotic susceptibility patterns of all patients with bacterial uropathogens will reduce multidrug resistance.

ISOLATION AND IDENTIFICATION of SOME BACTERIAL PATHOGENS FROM SUBCLINICAL MASTITIS of SHEEP in KIRKUK CITY, IRAQ

Almass.M. AL- bayati; Hala. M. Majeed; Ziyad. T. AL- doori

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 383-391

Background:- Mastitis is the term for a bacterial infection of the udder. It is most
common in ewes raising multiple lambs or with high milk production. Most cases occur
during the first weeks after lambing or immediately before weaning. Good ewe
nutrition and providing a clean lambing environment are important factors in reducing
the incidence of mastitis.
Objective: The study was focused on using of rapid & efficient methods for detection of
Subclinical Mastitis in 4 small holder dairy flock in central of Kirkuk &its affiliated
areas.
Material and Methods:- A total of 94 milk samples sheep were collected from four
farms in October to December 2018 to determine pathogens responsible for subclinical
mastitis in sheep.
Results:- Screening Subclinical Mastitis(of SCM) were done using California mastitis
and White Slide Test (WST). the prevalence percentage of SCM in California mastitis
and White Slide Test according the early stage lactation was(74 % and 70%)
respectively. While the prevalence percentage of SCM in California mastitis and White
Slide test according the according the dry period lactation was(66 % and 64 % )
respectively. identification of the isolates was achieved using Gram’s staining,
hemolytic pattern, colony morphology, Catalase , Coagulase test, IMVIC test and
confirmation of bacteria species by using Vitek 2 compact after identification to the
primary biochemical tests using Analytical Profile Index. Bacteriological examination of
all milk samples found the presence of (82.85% ) isolates where Staphylococcus was
predominant species (74.28 %) and the coagulase negative Staphylococcus (25.7% )
species was identified at the least bacteria .than staph. aureus, while environmental
pathogen represented E. coli second common pathogen followed by klebsiella
pneumonia (14.8% ) , Manhemia haemolytica (12.8 %) proteus spp.( 11.4%) And low
percentage to the Enterobacter spp. (4.3 %) .
Conclusion:- subclinical mastitis seems to be, as deduced from the high prevalence
observed in this study, an important health problem for milking sheeps in the Kirkuk
Application of VITEK system could be practiced at selected samples from time to time
to confirm identification of causative organisms.

Role Of Microbial Products In Dental Diseases: A Mini Review

Binay Sharma; Arun Karnwal

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 3016-3026

The oral cavity being the centre of array of organisms, endorses the establishment of diverse microbial associations like on the mucosa and teeth. Metabolism of these living beings encourages the attachment and development of the ensuing colonisers. A fragile adjust is kept up within the microbial ecosystem, with these life form conferring to normal growth and defences. Nevertheless, any change or disturbance within the microbial outline by reason of either outward or inborn components results unfavourable shift towards pathogenic organisms provoking numerous diseases such as dental caries or periodontitis. Moreover, current exposures also reveal that these microbes induce systemic illnesses such as diabetes or artherosclerosis. This article is an endeavour to confer an overview of the changed flora ailing states.