Online ISSN: 2515-8260

Keywords : SOD

Endogenous Antioxidant Activity Combination Of Moringa Leaf And Clove Flower Extracts Toward Diabetic Rats (Rattus Norvegicus)

Muhammad Fakhrul Hardani; Ririen Hardani; Sitti Rahmawati; Baharuddin Hamzah

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 1806-1813

In pathological conditions such as Diabetes Mellitus (DM), increased oxidative stress can cause a decrease in endogenous activity in the body so the body is unable to detoxify free radicals that cause cell damage. Some of natural antioxidant sources are moringa leaves (Moringa oleifera Lamk.) and clove flowers (Syzygium aromaticum Merry & Perril). The purpose of this study was to determine the enzyme activity of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) in a mixture composing of ethanol extract of moringa leaf and clove flower towards animals with DM conditions. This research is an experimental laboratory study using a post test only group design. The subjects of this study were 40 white male Wistar rats (Rattus norvegicus) strain which were DM conditioned by streptozotocin induction. Rats were randomly grouped into 8 groups, and each group consisted of 5 rats. The combined dosage of the ethanol extract of moringa leaves and clove flowers used is a combination I : 150: 40 mg / kg body weight rat, combination II 100: 80 mg / kg body weight rat and III 50: 120 mg / kg body weight rat. The results showed that the combination of ethanol extract of moringa leaves (Moringa oleifera L. and clove flower (Syzygium aromaticum Merry & Perril) increased the antioxidant enzyme activity of SOD and GPx in animals with DM conditions.

The impact of oxidative stress and some endogenous antioxidants on type 1 diabetes mellitus

Doaa Alaa Gheni; Jafar A. Al- Maamori

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4295-4310

Type 1 diabetes is one of the autoimmune diseases and is characterized by the destruction of beta cells in the pancreas, which leads to a complete loss or deficiency of the insulin hormone. This breakdown in beta cells is the result of several environmental, genetic, and oxidative factors are considered among the factors accused of destroying beta cells, so this study is designed to evaluate the effect of some oxidative factors in insulin dependent diabetes patients. The study includes 80 patients with type 1 diabetes compared with 40 healthy individuals as control group. The results showed a significant increase in the levels of total oxidant status (TOS) and malondialdehyde (MDA) and Transferrin in diabetic patients and a significant decrease in the levels of superoxide dismutase (SOD) and glutathione reductase (GR) comparing with healthy control. The study also noted over expression (folding change) for COX 7B in T1DM patients. The study concludes that oxidative status and enzymatic and non-enzymatic endogenous antioxidants play an important roles in the breakdown of beta cells, and thus it is one of the suggested factors responsible for developing type 1 diabetes mellitus.