Online ISSN: 2515-8260

Keywords : sephadex G-100

Equine Chorionic Gonadotropin (eCG) Polyclonal Anti Body Production in Madura Bulls for Estrus Synchronization and Superovulation Program in Ruminants

Herry A. Hermadi; Sunaryo H. Warsito; Erma Safitri

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 1263-1269

This study aimed to produce polyclonal anti-body (Abpo) equine chorionic gonadotropin (eCG) in Madura bulls. The Abpo-eCG was an alternative hormone for the superovulation technique and estrus synchronization of ruminant. The isolation of sera eCG derived from local Indonesian pregnant mare sera at 1-3 months age had beed collected from jugular veins, and then purification with charcoal was done by chromatography Sephadex G100. After that, the produce of Abpo eCG have done in two Madura bulls carried out injected with 3,000 IU eCG from local Indonesian pregnant mare sera 3 times at 10 day intervals. Furthermore, Abpo eCG was isolated from serum taken from the jugular vein of bull, then extraction was done by adding absolute charcoal and ethanol and centrifuged at 3000 rpm for 15 minutes at 4oC. Next step, the identification of Abpo-eCG that producted from Madura bull by SDS-PAGE 12% method, Western Blot and Elisa indirect to find out the highest level at the 7th week of 408.50 mlU /mL. Then purified by CM Sephadex G-100 coloumn chromatography technique. The final product was made as a Frozen Dry dosage form.

The Effects of Purified Staphylococcal Enterotoxin A on Gene Expression of Nuclear Factor Кappa B (NF-КB) in Rabbits

Ayat Ali Mahmood; Ahmad Darweesh Jabar; Muthana Ibrahim Maleek

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4319-4324

This study aimed to determine the effects of Staphylococcal enterotoxin A on NF-KB gene expression in rabbits. Firstly, SEA was isolate, purified and identified from one strain of Staph-ylococcus aureus collected from urine sample, and had identified by biochemical and molecu-lar methods. Then ,Staphylococcal enterotoxin A was purified by chromatography technique . All purified fractions of SEA was determined by ELISA kit. Finally, 5mg\ml of SEA was injected intravenously to rabbits in order to demonstrate its effects on NF-KB gene expression. At the end of this experiment, the gene expression of NF-KB increased to 9.1 in compression with control group with 1 of fold time (P = 0.045)