Online ISSN: 2515-8260

Keywords : Apoptosis


EUGENOL-LOADED CHITOSAN NANOPARTICLE INDUCES APOPTOSIS, INHIBITS CELL MIGRATION AND EPITHELIAL TO MESENCHYMAL TRANSITIONPROCESS IN HUMAN CERVICAL CANCER CELL LINE HELA CELLS.

Happy Kurnia P; Dhanang Puruhita T R; Muhammad Nazhif H; Rizq Threevisca C

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1184-1197

Eugenol is a phenylpropanoid group compound found in cloves, nutmeg,
cinnamon, and bay leaves. Apart from being used as a cosmetic, perfume, and food
ingredient, eugenol is known to have an antioxidant, antibacterial, anti-inflammatory, and
anti-cancer profile. Eugenol has therapeutic potential by increasing reactive oxygen
species formation, decreasing anti-apoptotic protein Bcl-2, increasing the release of
cytochrome c that leads to apoptosis in cancer cells, and inhibit the epithelial to
mesenchymal transition (EMT) process that could reduce the cell ability to migrating.
We synthesized eugenol loaded chitosan nanoparticles (Nano-EU) by ionic gelation
method to overcome its shortcoming which is volatile and to increase its bioavailability.
The nanoparticles were characterized by using Dynamic Light Scattering (DLS).
Anticancer activity of Nano-EU was investigatedin cervical cancer HeLa cell line by flow
cytometry using Annexin-V/PI staining, and by measuring cleaved-caspase-3 protein
expression which is the executor of the apoptosis process by immunofluorescence.
The results of the study evidenced that Nano-EU inducing apoptosis and increasing
activated caspase-3 expression in HeLa cells. Nano-EU could also inhibit cell migration by
reducing vimentin and Snail as mesenchymal markers leading to inhibition of the EMT
process. Further research is still needed to investigate the anticancer potential of Nano-EU
in HeLa cells to in vivo and clinical studies.

Cytotoxic Activities and Apoptosis Inducing Mechanism of Kiseueur (Antidesma tetrandrum Blume) Leaves and Bark in Breast Cancer Cell Lines (MCF-7)

Siva Hamdani; Chaidir .; Anas Subarnas; Doni Anshar Nuari

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4878-4883

Kiseueur (Antidesma tetrandrum blume) is an Indonesian native plant that is potential as an anticancer drug. This study aimed to determine the cytotoxic activities and apoptosis inducing mechanism of the extract and fraction of Kiseueur leaves and bark in breast cancer cell lines (MCF-7). The cytotoxic activity was carried out using the MTS assay. The expression of caspase 3 and caspase 9 pro-apoptotic proteins was observed by Western blot analysis. The results showed that the extract and fraction of Kiseueur leaves and bark had varied cytotoxic activities in breast cancer cell lines (MCF-7). Kiseueur bark extract had the highest cytotoxic activity against MCF-7 cells with IC50 81.65 μg / mL. Kiseueur bark extract induced apoptotic mechanism through modulation of caspase 9 and caspase 3 protein expression.