Online ISSN: 2515-8260

Keywords : lactobacilli

Characterization Of Exopolysaccharide From Lactobacillus Casei K7/3

Elova N. A.; Kutliyeva G.D .; Zakiryaeva S.I .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 506-515

An exopolysaccharide (EPS) in the amount of 20 mg/L and 1200 mg/L was obtained from the culture supernatant of Lactobacillus casei K7/3 grown in MRS broth and in a broth based on the whey of cottage cheese, respectivily. In the IR spectrum of EPS, intense absorption bands were found, generally characteristic of a class of carbohydrates. The molecular weight of the EPS obtained from L. casei K7/3 was 5600 Da, the polydispersity index was 1.9. The EPS from L. casei K7/3 consisted from a dextrose, mannose and galactose in molar ratio of 2.7:1.5:1, respectively. Minimal Inhibition Concentration (MIC) values of an EPS against Listeria monocytogenes ATCC 1911 revealed between 10 mg/mL and 80 mg/mL. An in vitro study of the antioxidant activity of L. casei K7/3 EPS in the diphenylpicrylhydrazine (DPPH) system showed that radical scavenging activity (RSA) has being increased depending on the concentration of the investigated EPS. The maximum %RSA was detected at 4 mg/ml being 26%.


Khaldarbekova G. Z.; Muhamedov I. M.; Tarinova M. V.; Shigakova L. A.

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 3183-3191

Scientists have been researching for a long time, where they have found evidence of a connection between “Lactobacillus - Caries”. According to the results of some countries, the qualitative and quantitative composition of lactoflora of the oral cavity is already known in the norm and case of diseases caused by disbiotic conditions of the mouth. This article presents the results of the quantitative and qualitative composition of oral lactoflora of women of fertile age, the state of biological properties of each particular representative and their impact on cavities formation, which can explain the microbiological etiology of cavities. Each of the following indicators was statistically processed and compared with the results of foreign countries.