Online ISSN: 2515-8260

Keywords : serotype

Molecular Characterization & Hematological Correlation Of Dengue Virus Among The Patients Attending Tertiary Care Centre, Jaipur, Rajasthan

Dr. Aditya Mishra, Dr. Ajay Kumar Gupta, Dr. Anshu SS Kotia, Dr. Shivani Shukla, Dr. Shivangi Shukla .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 1868-1873

Introduction: Dengue is arboviral diseases spread by the bite of infected Aedes mosquito carrying these viruses. Every year, thousands of individuals are affected and contribute to the burden of health care. However, there was little information on serotypes of dengue virus circulating in this part of the country. This study was carried out molecular characterization of Dengue virus isolated from patients in our area and study correlation of prevalent serotype with severity of disease along with the hematological parameters.
Materials& Methods: 1192 dengue like illness patient samples were collected from 2018 -2020 at JNUIMSRC, Jaipur. RNA extraction was done by Qiagen viral RNA mini kit & followed byRTPCR by using type specific primers & probes were used for Dengue RT PCR.
Results: A total of 1192 dengue like illness cases who had less than <14 days of fever, blood samples were collected from various departments of JNUIMSRC, Jaipur.  Out to 1192 cases 585 (49.07%) where Dengue was found. In Dengue Majority of the patients 161 males (27.52%) & 73(12.47%) females belonged to the 21-30 yrs of age group. Out of 585 cases total 108 (18.46%) were DEN I, 163 (27.86%) DEN II, 218 (37.26%) DEN III and 74 (12.64%) DEN IV and along with this 24-mix serotype infection was found.
Conclusion: Dengue remains to be an important health problem in India and across the globe.  There is neither any specific treatment nor any effective vaccine for dengue so far available in India. So, the proper diagnosis is the first requirement for the management of the disease. DHF &DSS are life threatening. Molecular method is much needed as it hardly takes 4 hrs. To produce accurate result

Genotype Variants Of Dengue Virus On Dengue Hemorrhagic Fever (Dhf) Suspect: Cross Sectional Study In Health Facilities In Semarang City, Indonesia

Martini Martini; Syahilla Efriana; Rini Fajarini; Ari Udiyono; Retno Hestiningsih; Atik Mawarni; Indranila Kustarini

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 730-740

Semarang has been reported as an endemic area of dengue hemorrhagic fever (DHF), yet continuous data of molecular epidemiology related to dengue fever is not available. The aim of this study was to analyze the characteristics of virus strains, host suspected dengue, and the dynamics of dengue fever in Semarang City. The design of this study was observational description with cross-sectional design. Seventy three DHF suspected patients from two hospitals and seven health centers in Semarang City were sampled during 2017- 2018. Reverse Transcription Polymerase Chain Reaction (RT-PCR) method was used to examine serum suspected dengue and sequence serotypes and genotypes of dengue virus. The results showed that the highest frequency to be exposed by DHF was infant and children; while, the number of male and female with suspected dengue was relatively the same. The dominant serotype was DENV-3, DENV-1, and mix of DENV-2 and DENV-3; while, the circulation of DENV-1 genotype I and DENV-3 genotype I were identified as Indonesian local endemic strain causing epidemics in Indonesia.