Online ISSN: 2515-8260

Keywords : Hydrogen peroxide


The ability of astaxanthin-rich Haematococcus pluvialis algal extract on preventing harmful effects of H2O2 on human fibroblast cells in vitro

Quan Minh To; Dieu Quang Tran; Tuyet Thi Vy Le; Ha Le Bao Tran; Long Thanh Le

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 1809-1819

This study aimed to determine the ability of astaxanthin-rich Haematococcus pluvialis algal extract (AE) on preventing adverse effects of H2O2 on human skin fibroblast cells. A Haematococcus pluvialis strain obtained from Vietnam was used as a source of astaxanthin. hF cells were cultured in DMEM/F12 medium supplemented with AE 0, 10, 25, 50 μg/ml for 7 days. WST-1 assay results showed that AE 10-50 μg/ml was not poisonous and didn’t have any negative effects on cell proliferation. hF cells were pre-treated with AE (0, 10, 25, 50 μg/ml) for 24 hours prior to H2O2 treatment (200 μM, 120 minutes). The results showed that AE protected hF cells: reduction of G0/G1 phase cells in cell populations (16.08 – 21.52%), senescence-associated β-galactosidase – positive cells (28.9 – 40.8%), and maintenance of cell proliferation, nuclear area. AE 10 μg/ml is the optimal concentration in this study. In conclusion, AE has been shown to have the ability to protect fibroblasts from the adverse effects of H2O2.

A comparative analysis of different bleaching agents in primary and permanent teeth

Dr. Lavina Bhatnagar; Dr Ekta Choudhary; Dr. Priyadarshani Suryawanshi; Dr. Sagrika Grover; Dr. Ankita Bhargava; Dr. Raksha Thakur

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 7890-7895

Background: The intrinsic stains can be treated by various measures such as internal bleaching of nonvital teeth, external bleaching of vital teeth. The present study was conducted to compared carbamide peroxide (CP), sodium perborate (SP) and hydrogen peroxide (HP) as bleaching agents in primary and permanent teeth.
Materials & Methods:
Results: The mean color value at day 0 in sub group I A was 9.6, in group IB was 10.4 and in group III A was 11.5, in sub group II A was 10.6, group II B was 10.7 and group III B was 11.3. The difference was non- significant (P> 0.05).The mean color value at day 0 in sub group I A was 3.9, in group IB was 6.8 and in group III A was 9.4, in sub group II A was 8.4, group II B was 8.7 and group III B was 8.8. The difference was non- significant (P> 0.05). The mean color value at day 0 in sub group I A was 2.7, in group IB was 6.5 and in group III A was 8.5, in sub group II A was 7.0, group II B was 7.8 and group III B was 8.3. The difference was non- significant (P> 0.05).
Conclusion: All bleaching found to be equally efficient in discolored primary teeth whereas CP was effective in discolored permanent teeth.

Comparative Quantitative Characteristic of Inflammatory Reaction After Pleurodesis Using Solutions of 4% Sodium Bicarbonate and 6% Hydrogen Peroxide in the Experiment

Vladimir F. Kulikovsky; Alexander F. Сhernousov; Svetlana G. Gorelik; Michail S. Izyumov; Victor V. Bulynin; Evgeny F. Cherednikov; Andrey M. Bobrovskikh; Igor V. Yurgelas

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 5608-5614

The purpose of this study is to perform a comparative analysis of the quantitative characteristics of the inflammatory response in the tissues of the lungs, pleura and adjacent subpleural areas of the chest wall to chemical pleurodesis using solutions of 4% sodium bicarbonate and 6% hydrogen peroxide in the experiment. An experiment was performed on 150 experimental animals (rats) in which spontaneous pneumothorax was modeled. Subsequently, after 1 hour, a solution of 4% sodium bicarbonate or 6% hydrogen peroxide was sprayed. The calculation of key statistical parameters of the presented data was carried out using descriptive statistic methods. The studied samples were evaluated using the Kruskal-Wallis test. Differences in the compared samples were considered significant at p ≤ 0.05. In both comparison groups, the predominance of neutrophils in the first days of the experiment was revealed, which indicates an acute inflammatory reaction to the chemical agent introduction. In the following days of the experiment, a decrease in the number of neutrophilic leukocytes was observed, simultaneously with an increase in the number of lymphocytes, macrophages, histiocytes, which indicated the transition of acute to chronic inflammation. It was established that the pleurodesis method has a statistically significant effect on the number of free cellular elements involved in the inflammatory reaction, despite the stereotype of the very dynamics of free cellular elements in all comparison groups. During chemical pleurodesis using a 6% hydrogen peroxide solution, the signs of acute inflammatory reaction from the pleura and the adjacent chest wall areas in experimental animals are shorter in time and not so intense compared to the similar group where a solution of 4% sodium bicarbonate was used.