Online ISSN: 2515-8260

Keywords : serology

To study the seroepidemiology of measles at a tertiary care centre

Dr. Meda Shailaja Rani, Dr. P Tejaswi Sai, Dr. V Sudha Rani, Dr. P Shashikala Reddy

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 5, Pages 61-70

Back ground: The "gold standard" for diagnosing an MV infection in a lab is the presence of particular immunoglobulin M (IgM), which can be detected using either an ELISA (enzyme-linked immunosorbent assay)or immunofluorescence. A most popular type of testing is serological because it is quick, affordable, and reliable and can be done in a high-throughput setting. According to earlier research comparing IgM tests, with RT-PCR-based assays as a reference, sensitivities ranged from 89.9 to 98.8% and specificities ranged from 92.5 to 97.9%.
With this background, this study was conducted to serologically diagnose clinically suspected cases of measles by IgM Antibody detection in our setting and also to correlate measles infection with vaccination status.
Materials and Methods: We included all patients aged less than 24 years belonging to both genders visiting the Paediatrics/Medicine OPD at Sir Ronald Ross Institute of Tropical and Communicable diseases, Nallakunta, Hyderabad, during March 2019 to March 2020. The sample size considered was 100. ELISA kit with IgM for measles was used to serologically diagnose measles in suspected cases.

Analysis of the Dengue Infection, Occurrence and Hematological Profile of Dengue Patients in Dhaka City

Mohammad Zakerin Abedin; Md. Sifat Uz Zaman; Tasnim Ahmad; Md. Abdullah Al Maruf; Md Babul Aktar; Md. Fayez Ahmed; Md. Abu Sayeed Imran; Rubait Hasan; Pinki Akter; Md. Ekhlas Uddin

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 1572-1578

In the tropical and subtropical area, dengue virus is mostly found. To the determination of the occur-rence of dengue viral infection and hematological profile of dengue infected peoples in the Dhaka city. A sum of 255 samples of blood from both sex and different age groups were collected in this research. Dengue nonstructural protein 1 (NS1) and complete blood count (CBC) test were performed for the study. During this study period, total 255 samples were tested. Among these, 67samples were diagnosed as positive. Seroprevalence of dengue was 26.30%. Out of 67 positive dengue patients, 41 (61.2 %) were male and 26 (38.8 %) were female. Dengue infection was observed more in 21 to 30years age group followed by above 40years and 16 to 20years but observed less in of 1st day to 5years followed by 11 -15years, then 6 -10years and 31 - 40years. It was seen that the >40years of age group had the maximum unit of cases having low platelet count followed by 21-30years and it was also seen that 21-30years of age group had the maximum range of cases having low WBC count followed by above 40years of age group. In our research, we showed thrombocytopenia (100000 - 150,000/μl) with leucopenia (White Blood Cells, WBC <5000/μl) in 8(26.6%) cases and thrombocytopenia (100000-150,000/μl) without leucopenia in 9 (24.3%) cases but thrombocytopenia (<100000/μl) with leucopenia (White Blood Cells (WBC) < 5000/μl) in 5(16.7%) cases and thrombocytopenia (<100000/μl) without leucopenia in 6 (16.2%) cases. Among 67 positive cases, we found that (26.6%) had DF and (16.7%) had DHF according to WHO classification. For validating more reliability, this research needs further work.

Molecular Diagnostic Methods For Viral Infections

Dhakshinya M; Smiline Girija A.S; Ezhilarasan D

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 2595-2605

Viral diagnostic tests are categorized as direct diagnosis, indirect examination (virus isolation), and serology. Molecular testing is often done to detect potent viral markers resulting from any gene or chromosomal modifications during viral replication in the host. The clinical specimen is tested specifically in direct analysis for the presence of virus particles, virus antigens or viral nucleic acids. Molecular diagnosis of viruses can be achieved from clinical samples like blood, hair, skin, amniotic fluid, or other tissue. Molecular biology techniques are considered to be the standard and confirmatory protocols in the analysis of viral DNA, RNA and protein. Molecular viral diagnosis are considered to be more specific and sensitive in preliminary viral screening and can pave the way for rapid diagnosis. However, the procedures are costly and involve automated bio-instruments to perform the same. This review thus highlights the various molecular diagnostic methods used in diagnostic virology with their limitations and challenges.