Online ISSN: 2515-8260

Keywords : Spike protein


Antibody response in healthcare workers to COVISHIELD vaccination in a tertiary care hospital

R Mahesh Reddy, Yasmin Muhammed, Kundan Tandel

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 1933-1939

Background: SARS CoV-2 infection has become a major public health concern. India started Covid-19 vaccination from January 16, 2021, after the approval of two candidate vaccines namely COVISHIELD ™ and COVAXIN ™. The present study was conducted to see the neutralizing antibody response to trimeric S protein of SARS CoV-2 in health care workers (HCWs) with 2 doses of COVISHIELD vaccination in a tertiary care hospital.
Methodology: A prospective cohort study was conducted among 156 healthy adult health care workers in a tertiary care centre, vaccinated during January-March 2021. They were divided into two groups, the first group comprised of individuals who were previously RT-PCR positive (n=36) for SARS CoV-2, and second group comprised of those who were RT-PCR negative (n=120). Blood samples were collected from all participants, the first sample on the day of vaccination, second sample after 4 weeks of vaccination, and third after 8 weeks of vaccination to measure the IgG antibodies against the SARS CoV-2 ‘S’ protein using a chemiluminescent quantitative immunoassay.
Results: The spike protein-specific IgG antibody titre was demonstrated reactive cut-off in 98.3% of the participants after 2 doses of vaccine. The median antibody titre declined from 710.5 (IQR, 338.5-1577.5) to 266 (IQR, 116-557.75) in RT-PCR positive HCWs after 8 weeks of vaccination whereas it increased from 45.1 (IQR, 31.475-76.575) to 83.4 (IQR, 52.075-104) in RT-PCR negative HCWs.
Conclusion: We could demonstrate the development of an adequate spike protein-specific IgG titre against SARS CoV-2 following vaccination with 2 doses of COVISHIELD in HCWs.

ImmunobiologyOf N-Cov Spike Protein

M. Rithanya; Smiline Girija; M.P. Brundha

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 2965-2972

Immunobiology is the branch of science that deals with immunological effects which includes phenomenan like infectious diseases, growth and development, hypersensitivity, heredity, aging and transplantation. In this review article, we have compiled the immunobiology of the nCoV, which has led to the world wide panic causing a major pandemic. Coronavirus (CoV) is a large family of viruses that has led to mild to severe illness, with magnified cytokine storms in the lungs. The severe forms of these viruses involve Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome (SARS-CoV). A new strain of virus that had not been earlier detected in humans is the Novel coronavirus (nCoV). The major structural protein, the spike protein S seems to be the highly potent moiety involved with viral fusion and entry into the host cells through ACE receptors. This review thus throws insights into the immuno-biology of the spike protein and its role in the immuno-pathogenesis of CoV.