Online ISSN: 2515-8260

Keywords : Microscopy

Comparison of Microscopy and Polymerase Chain Reaction Examination Results in the Detection of Malaria Parasites

Nailatul Hana; Lia Faridah; Hesti Lina Wiraswati; Jontari Hutagalung

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 2871-2881

Background: Microscopy remains the mainstay method for malaria diagnosis worldwide, although species misidentifications have been detected in practices due to various limitations, such as hypnozoites detection and lower parasitemia in asymptomatic malaria. Polymerase Chain Reaction (PCR) is a molecular diagnostic method with high accuracy in detecting species of organisms. This study was aimed to evaluate the diagnostic performance of microscopy compared to nested PCR in detecting malaria parasites.
Methods: A cross-sectional study was conducted with previous data on malaria assessment in East Nusa Tenggara. More than 500 asymptomatic respondents were included by the systematic random sampling method from 5 sub-districts area in the region based on API. Microscopic assessment by thick and thin blood smears was made following protocols from the Ministry of Health, while DNA isolation was done using 200 μl fresh blood sample and nested PCR amplification protocol with specific primers of the malaria parasites species Plasmodium sp.
Results: A total of 555 specimens were collected, and 1.6% (9/555) of those were microscopy-positive and 32.6% (181/555) were detected positive by nested PCR. Of microscopy-positive samples, 33.3% (3/9) were P. falciparum and 66.7% (6/9) were P. vivax, whereas among PCR-positive samples, 31.5% (57/181) were P. falciparum, 52.5% (95/181) were P. vivax, and 16.0% (29/181) were mixed infection of both species. From this study, microscopy was found to had a slight measure of agreement (κ = 0.055) compared to nested PCR.
Conclusion: In lower parasitemia and asymptomatic malaria, the microscopic assessment may not be sensitive. Thus, this increases the need of using PCR assessment to confirm the identification of malaria parasites.

Experimental Estimation Of "Denta Aloe" Efficiency In The Complex Treatment Of Sutton’s Aphthae

Khaydar Kamilov; Shakhnoza Khodzhiakbarova; Kamolakhon Takhirova; Aliya Kadirbaeva; Farangiz Bakhramova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 5280-5292

The problem of diseases of the oral mucosa covers a wide range of issues. Despite numerous studies, etiology, pathogenesis, clinical presentation, treatment of this pathology is not fully permitted.
The current most widely used medical term is "recurrent aphthous stomatitis" or simply "aphthous stomatitis". Historically, many different terms have been used to refer to recurrent aphthous stomatitis or its sub-types, and some are still in use. Synonyms for major RAS include Sutton's ulcers (named after Richard Lightburn Sutton), Sutton's disease, Sutton's syndrome and pariadenitis mucosa necrotica recurrens. The incidence of the disease ranges from 10 to 40%, according to WHO, 20% of the population is affected. Currently, the etiology and pathogenesis of this disease are not fully established and, despite the variety of studies conducted in our country and abroad, remain the subject of numerous discussions. Microscopic and histological studies of the " Denta Aloe" preparation were carried out in the histological laboratories of the Republican Pathological Center, the staining of the micropreparations was performed using hematoxylin and eosin dyes.

Scanning Acoustic Microscope (Sam)- A Review

Karishma Desai; Leslie Rani. S; Brundha MP

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3129-3136

Scanning Acoustic Microscope, known as SAM, is a device that is used to investigate, measure or image an object. It is widely used for a non-destructive evaluation which is its biggest plus point. A Scanning acoustic microscope is useful for detection of defects in multilayered structures by a low time consuming process. The aim of this review is to understand the applications, uses and structure of this microscope. A systematic search strategy was employed and articles were found using keywords. Literature was taken from databases like PubMed and Google Scholar. Articles that discussed the use of SAM and its uses were included. Other articles which had data regarding the structure and its applications in different fields were also included. A total of about 100 articles were collected initially. Multiple articles were added later from other sources. After eliminating articles that did not meet the inclusion criteria, more than 50 studies were finally obtained. This review summarized the use of a Scanning Acoustic Microscope. Its structure, and other applications among different fields were also discussed in detail.