European Journal of Molecular & Clinical Medicine

Nowadays, there is a continuous and progressive global public exposure to acrylamide (ACR) due its presence in fried and backed food. Thermal preparation of sugar-rich food mostly initiates chemical reactions that yield dangerous chemicals. Of these chemicals arise acrylamide and its metabolite glycidamide. Those having a high
affinity for binding to nervous tissues. The acrylamide-induced neurotoxicity involves reduction in proliferation of neuronal progenitor cells associated with apoptotic cell death. Concerning acrylamide-induced toxicity, preventive medicine research focused on natural antioxidants. Studies on active constituents of Saffron showed obvious antioxidant, and anti-toxic properties. Here, we designed this study to investigate the possible protective
effect of saffron against acrylamide-induced neurotoxicity in hippocampal area of brain. Adult male albino rats were assigned equally into 4 groups (n = 8); control; saffron group; acrylamide (ACR)-administered group and combined ACR and saffron- treated group. Our results recorded molecular changes as a significant decrease on genomic DNA fragmentation in saffron co-administered group as compared to ACR-exposed group. The changes on DNA mutagenicity were studied by analyzing the random amplification of polymorphism of DNA that showed an elevated genomic integrity on rat co -administered saffron with ACR in contrast to those exposed to ACR only.

Recently parents want their children to be busy with a particular kind of sport. Adults often decide on this issue, based on their own desires. But still, we must remember that, first of all, it is necessary to focus on the child's abilities and capabilities. Physical culture and sports are different things, and they pursue different goals: they are engaged in physical culture in order to strengthen their physical condition and be healthy, and sports are also for the sake of victories, achievements, and results. In humans, more than two hundred genes are known that are associated with endurance, speed and strength. Usually, the reason for differences (polymorphism) of genes is the replacement of individual nucleotides in the DNA molecule, leading to a change in the properties of the gene.
The main advantage of molecular genetic methods is that a person’s behavioral activity is shown to be hereditary, and early diagnosis reveals a highly informative assessment of the possibility of physical development. A distinctive feature of this diagnosis is the ability to identify hereditary trends, the development of factors of occupational diseases, determining the physical performance of a person and reducing the quality of his life.

In the present study the extraction of DNA from Polyporusmonticola and Trichodermaviride and amplify it using PCR to ascertain its purity and it was carried out using two wood decay fungiPolyporusmonticola and Trichodermaviride. It was concluded that the powerful fermation effect was attributed to the greater amount of DNA obtained from various extracts of Polyporusmonticola and Trichodermaviride.