Online ISSN: 2515-8260

Keywords : congenital anomalies


ROLE OF TORCH INFECTION, OXIDATIVE STRESS AND SOMATIC DNA DAMAGE IN HIGH-RISK PREGNANT WOMEN LEADING TO CONGENITAL ANOMALIES

Swapna C Senan; Anitha C; Sarala Gopalakrishnan; Dinesh Roy D

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 1125-1132
DOI: 10.31838/ejmcm.07.09.117

An increased complication rate during or after pregnancy and birth leads to a condition known to the mother or child when High Risk Pregnancy (HRP) and may be for both. An attempt was made here to find the role of TORCH infection in high-risk pregnant women; oxidative stress and somatic DNA damage, leading to congenital anomalies(CAs).The patients were referred from various infertility clinics and maternity centers of Kerala to Advanced Genetic Study Centre (Genetika), Trivandrum, Kerala. Study population includes 170 study subjects and 105 healthy controls. Blood sample was collected for TORCH IgG (ELISA), oxidative stress evaluation (Malondialdehyde by thibarbituric acid assay) and somatic DNA damage (CBMN assay). Among 275 subjects, 170 (61.81%) had the history of high-risk pregnancy with a mean age of 35.65±6.30 years. The control group included 105 women with previous normal pregnancy with a mean age of 34.03±6.10 years. TORCH infections had a significant role in HRP (p-value<0.001). Study group population showed a mean MDA value of 2.95 ± 2.09 and that of control group was1.67 ± 0.70. The mean value of CBMN frequency was 12.44 ± 1.01 in study subjects and that of controls was 9.98 ± 0.58. Study group showed a higher CBMN frequency and MDA value than control group. Proper diagnosis and intervention during early stage can be done by routinely screening of sample for TORCH agents, oxidative stress and somatic DNA damage even if the case is asymptomatic which shows high risk in postnatal periods. This can manage and reduce adverse foetal outcomes efficiently, thus reducing morbidity and mortality.

Prevalence and Risk Factors of Congenital Anomalies among the sick neonates of the Ladakh region of India

Mohd Murtaza; MdNiamat Ali; MahrukhHameed Zargar; Oliyath Ali

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 1083-1092

Congenital anomalies are the structural and functional irregularities at birth. In this progressive study, all the neonates who are admitted to the Neonatal Intensive Care Unit during the three year period from 1st June 2017 to 31 May 2020.
Aim: The study aims to examine the prevalence, type, and epidemiological factors of congenital anomalies among the population of Ladakh India.
Subject and methods: All the neonates during the study period were examined by pediatrics and questionnaire filled by the consent of the family. A total of 936 parents of neonates was agreed to participate in this study and among them, 524 are male neonate and 412 was female.
Results: Four and a half percent were diagnosed as being congenital malformed and the common system affected by congenital anomalies in the digestive system followed by the mucso-skeletal system. In this study, based on the logistic regression consanguineous marriage with OR of 9.862 (4.221; 23.041), P= <0.001, familial congenital anomalies in the family with OR of 17.008 (4.343; 66.606), P=0.001, an anaemic mother with OR of .124 (.029; .538). P = .005, Apgar score with .033 (.016: .067), P-value of <0.001 and paternal smoking with OR of 13.305 (5.558: 31.854) with P value of <0.001 had shown a very good significant correlation with the congenital anomalies.
Conclusion: The occurrence and distribution of congenital anomalies in Ladakh were reported. More active prenatal screening and the possible role of genomics study are major importance to uncovering the susceptibilities.