Online ISSN: 2515-8260

Keywords : Bone

“Comparison Of Conventional Decalcification Method And Microwave Decalcification Of Bone”-A Reaserch Protocol

Sahitya Vodithala; Yugeshwari R. Tiwade; Sunita Vagha

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 3880-3886

Background:Bone “decalcification” is a crucial step during the tissue processing in day to day histopathology section. So as to achieve good paraffinized sections of bone, the calcium which is in inorganic form should be removed from natural matrix which is made up of collagen,the cartilage which has been calcified and neighboring tissue material. The process is popularly known as "Decalcification". It is completed through the meansof chemical retailers such as i)various acids which produces calcium salts and these salts are soluble and ii) chelators that combine with ions of calcium.
Post decalcification,the collagen which is present in cortical bone remains to be harden greater after paraffin tissue processing. The preference of decalcifier is inspired by 4factors which are dependent on each other -case emergency, mineralization process, quantity of the examination, and various stains which will be done.
Acids which are perfectly buffered, cause few adverse outcomes on tissue stain-dryness. This difficulty will increase with acidity of solution, decreased pH, and durationof decalcification. As a result, the speedy decalcifier have abad effect on successive staining if incompletely fixed. So, the chromatin which is present in the nucleus fails to grasp hematoxylin and numerous dyes which are basic. Acidic dyes staining effects less comparatively, however eosin which is an acid dye will stain the tissues intense, troublesome and red. These after-effectsmay be decreased by performing „end point test of decalcification‟, „removal of acid post decalcification‟and „regulating staining methods‟.
Objectives:To study the comparison of conventional decalcification method and microwave decalcification method of bone by using 10% Nitric Acid with regards to decalcification speed, conservation of the tissue architecture as well asstaining productiveness.
Methodology: In the presentstudy, both methods of decalcification i.e; microwave method and conventional method are done in a sample size of 30 hard tissue specimens received in Division of Histopathology, Department of Pathology. The results of these two methods will be compared with regards to decalcification speed, conservation of tissue architecture and staining productiveness.
Expected Results – Results will be evaluated after the completion of the study.
Conclusion- The conclusion will be based on the results of study.


Chandrakala B; Govindarajan Sumathy; Bhaskaran Sathyapriya; Pavishwarya P; Sweta Jain

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 1662-1667

Sella turcica is a saddle shaped bony structure present on the sphenoid bone. The pituitary gland is seated at the inferior aspect of the sella turcica, called hypophyseal fossa. Sella turcica serves as a cephalometric landmark, that being said any morphological changes can affect the overall craniometry of the individual as well as alter the function of the structures it lodges. The following review emphasis on the possible morphological changes of sella turcica and its effects on the individual.