Online ISSN: 2515-8260

Keywords : Multiple Sclerosis

Role Of Lincrna-P21 And Lncrna-H19 In Multiple Sclerosis Disease

Laila M. Meber; Olfat G. Shaker; Amr H. El-Sayed; Nermien E. Waly

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 429-442

Multiple sclerosis (MS) is a chronic immune-mediated central nervous system (CNS) disorder with several environmental and genetic factors. To participate in the regulation of immune responses, long non-coding RNAs (lncRNAs) have recently been published. As a result, aberrant expression of lncRNAs has been proposed as an underlying cause of MS. In the current research, by means of quantitative real-time polymerase chain reaction (PCR), we assessed the expression levels of two lncRNAs with putative functions in the regulation of immune response, namely lncRNA-H19 and lincRNA-p21, in serum of 74 Egyptian patients with MS relative to healthy people. Significant downregulation of lncRNA-H19 and lincRNA-P21 expression levels relative to controls in the serum of MS patients was observed (P < 0.001). Correlation analyses of lncRNA expression levels and MS patient clinical data showed a substantial moderate positive linkage among lincRNA-p21 serum expression levels and the Expanded Disability Status Scale (EDSS), a substantial moderate negative linkage among lncRNA-H19 expression levels and MS onset age and no substantial correlation among these lncRNAs and patient age. Furthermore, we showed no significant correlation among lncRNA-H19 and lincRNA-p21 serum expression levels. In brief, in MS patients, we have shown dysregulation of two lncRNAs. To explore the precise mechanisms through which lncRNAs engage in the regulation of immune responses, more studies are required

Hotair (Homeobox Antisense Intergenic Rna) And Gas-5 (Growth Arrest–Specific 5) Expressions As Biomarkers For Early Diagnosis Of Multiple Sclerosis (Ms)

Marwa M Kamel; Shaker O; Amr Hassan; Nermien E. Waly

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 561-576

Background: Multiple sclerosis is a central nervous system autoimmune illness that is characterized by chronic inflammation, gliosis, demyelination, and neuronal loss. Long noncoding RNAs (LncRNAs) play a significant role in regulating immune response, as well as the development of immune cells.
Objective: This study aims investigate the role of lncRNAs; HOTAIR (HOX transcript antisense intergenic RNA) and (HOX transcript antisense intergenic RNA) in MS pathophysiology and their impact on clinical course of the disease.
Patients and methods: The present study was conducted on 134 subjects; 74 patients with MS (relapsing remitting and secondary progressive types) and 60 age and sex matched controls. Expression of both lncRNAs; HOTAIR and GAS-5 was assessed in serum using Real-time quantitative PCR (qPCR). The clinical disability in the patients was evaluated using the Expanded Disability Status Scale (EDSS) at the time of patient enrollment.
Results: The relative expression levels of HOTAIR were significantly down-regulated in serum samples of MS patients relative to the control group; (p value < 0.001) while relative expression levels of GAS-5 were significantly up-regulated in serum samples of MS patients relative to the control group; (p value = 0.002). There is significant positive association between GAS-5 expression level and age of onset in MS patients (P value= 0.020).
Conclusion: This differential expression of both lncRNAs may have an important role in MS pathophysiology. This study clarified the molecular pathways through which those lncRNAs contribute to MS clinical presentation.

Need of Comprehensive Physiotherapy in Multiple Sclerosis: A Narrative Review

Archana Choudhary; Dr. Amandeep Singh

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4754-4761

Multiple Sclerosis is a chronic progressive and debilitating disease of adults which attacks myelin sheath in brain and spinal cord, leading to problems with coordination, balance, spasticity, muscle control, disability, gait disturbances and other basic bodily functions. The course of disease is highly unpredictable which greatly vary among MS patients. In majority MS attacks young adults which significantly affect the quality of life at an early stage of life. As far there is no known cure has been established for MS and patients are profoundly depends on DMDs to improve their physical as well as mental symptoms although the treatments are very expensive. Patients with MS usually do not involve in physical activity due to afraid of worsening of pre-existing symptoms or might get a relapse. Physicians now believe that exercises can play a crucial role in preventing deconditioning and to improve physical and mental symptoms as well as may have possible neuroprotective role in MS. This article reviews the effects of Physical therapy interventions on pathophysiology and on specific impairments in MS patients, and emphasizes a productive role of physical rehabilitation in PwMs.
A comprehensive literature search was done by formulating the research questions such as effects/role /benefits of exercises in MS (PubMed, SweMed, NCBI, Cochrane, Elsevier, Wiley online Library, Hindawi)