Online ISSN: 2515-8260

Keywords : colon cancer


Tecoma stans Hydroethanolic Leaf Extract its Antioxidant and Cytotoxic Activity Against Colon Cancer (HT-29)

Priyanka Sivasubramanian; Lavanya Prathap; Selvaraj Jayaraman; Preetha. S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 550-559

BACKGROUND :-
Colon cancer is known to be the third most deadly and fourth most commonly diagnosed cancer in the world. In this research we study the anti-cancer properties of the plant leaf extract of Tecoma stans. There has always been a need for anti-cancer agents, since the commercially available synthetic drugs have their own limitations due to undesirable side effects. AIM :- To analyse the antioxidant and cytotoxic activity of Hydroethanolic leaf extract of Tecoma stans in colon cancer cell lines.
 
MATERIALS AND METHODS :-
Human colon cancer cell line (HT -29) was brought from NCCS, Pune, India. Cell viability test and Gene expression analysis were carried out for P-I3 and Akt mRNA Gene expression using MTT and PCR respectively. The results were analyzed using appropriate statistical tools.
RESULT :-
Results suggest that maximum reduction of cell proliferation was at dosage of (100-500 μg/ml) of the Hydro ethanolic leaf extract of Tecoma stans used in the study when compared to untreated group. At doses of 300 g/ml and 400 g/ml, the cancer cells were severely suppressed and mRNA expression of P-I3 and Akt was significantly reduced as compared to control.
 
CONCLUSION :-
The research carried out revealed that leaf extracts of Tecoma stans have diverse pharmacological spectrum and contain anti-cancer behavious. Research on this plant is increasing day by day because of its potent pharmacological uses. However further investigations involving the isolation on screening models of constituents are needed for further confirmation of various potential of Tecoma stans

The effect of exercise-induced dopamine replica in colon cancer cell line (HT-29) prevention via apoptotic signaling pathway

N.Vishal Prakasam; Lavanya Prathap; Selvaraj Jayaraman; Preetha. S

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 8, Pages 706-715

During exercise we release a lot of hormones and neurotransmitters. Dopamine is one among the hormones which is released in several parts of the mid brain during exercise. It is said that exercise can help to avoid many diseases and even cancer.. In 2020 the number of cases diagnosed were 104,610 all around the world .In this study we analysed the role of endogenous dopamine replica over gene expression of Apoptotic and tumor suppressor gene against colon cancer prevention.
Human colon cancer cell line (HT -29) was brought from NCCS, Pune, India. Cell viability test and Gene expression analysis were carried out for apoptotic and tumor suppressor gene using MTT and PCR respectively. The results were analyzed using appropriate statistical tools using ANOVA and Duncan’s test.

In-Vitro evaluation of anti-cancer and genotoxic potential of medicinal herb Saussurea lappa extract in human cancer cell lines

Ayyub A. Patel; Mohammed Amanullah; Fahmy G. Elsaid; Tamer Soliman; Mohamed Eissa; Hani Alothaid

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 736-747

For centuries, plants are known to play part in the daily routine from providing food to the management of human health. During the last few decades, diverse phytochemicals have been characterized as agents that possess a potential to execute cancer cells. The oil extracted from the root of Saussurea costus; has been used in traditional medicine for a long time. Purpose: This study was carried out to assess the effect of Saussurea lappa aqueous extract on five different cell lines; Human Lung Fibroblast cells (MRC5), Human Dermal Fibroblast adult (HDFa), Madin-Darby Canine Kidney cells (MDCK), breast cancer cells (MCF7) and human colonic cancer (Caco2). Results: On normal cell lines; the extract showed cytotoxic activities with the half maximal inhibitory concentration; IC50 values, ranging from 0.85 to 2.5 mg/ml whereas MRC5 was the most sensitive cell line (IC50 values: 0.85). On malignant cell lines; MCF7 cells showed inhibition in cell proliferation with activity from 67.17% to 91.01% by the extract treatment concentration 0.50 to 2.0 mg/dl. However, Caco2 cells exhibited inhibition in proliferation ranging from 37.10% to 69.45% by the same treatment dose. Thus, breast cancer cells were more sensitive than colon cancer cells to Saussurea costus extract. The genotoxic potentialities of the crude extract showed increase in expression of proapoptotic genes; P53, IκBα, BAX and TNF and decrease in the expression of antiapoptotic genes; Bcl2, Survivin, and MMP-7. Conclusion: Our work pointed out that Saussurea lappa extract could be a potential candidate that has the capability to fight cancer.