Online ISSN: 2515-8260

Keywords : angiogenesis

Immunohistochemical Study Of Angiogenesis In Giant Cell Tumor Of Orthopedic Patients

Dr. Maha Khadum Ghayadh

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 7, Pages 2862-2873

Objectives: To study the angiogenic activity in giant cell tumor of  bone, by means of VEGF expression, in the endothelial cells using immunohistochemical staining.
 Material and methods: A cross sectional study was conducted from March 2020 to April 2022. 50 cases of GCT of bone were included in this study. Tissue sections that were retrieved from archived tissue blocks were stained with immunohistochemical stain for VEGF protein.
Results: Our study revealed that thirty nine of the cases showed a positive staining for VEGF in the tumor cells (72.2 %). There was a significant association between the expression of VEGF in the tumor cells with tumor stage (p=0.003). There was also a significant association between the expressions of VEGF in the tumor cells with the presence of pulmonary metastases (p=0.015).
Conclusion:  GCT of bone is frequently hypervascular; VEGF secreted by tumor cells elicits angiogenesis, which significantly contribute to the development of pulmonary metastasis. This provided confirmation for the prognostic significance of VEGF in predicting the behavior of GCT of bone and also the basis for therapeutics strategy targeting angiogenesis.

VEGF-A, VEGFR-2 and MVD in brain tumor tissue

Agnieszka Nowacka; Wojciech Smuczyński; Maciej Śniegocki; Kamila Woźniak- Dąbrowska

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 1180-1187

Neoangiogenesis plays a crucial role in tumor development. The method that is most commonly recommended for assessing this process is determining the so-called microvessel density (MVD) in the tissue sample. In relation to tumors of the central nervous system, it seems that VEGF-A along with its receptor VEGFR-2 are the most significant factors, and the important role of both of them is connected with their increased expression in tumor tissue. The study concerned 48 adult patients of both genders, treated surgically for newly diagnosed solid brain tumors andconsisted of histological assessment of the material gained from the surgically removed brain tumor. The highest level of VEGF in tumor tissue was observed in gliomas – mostly HGG - 12.61 (N = 23, p = 0.000059). As for VEGFR-2 in tumor tissue, the highest average level was characteristic for metastatic tumors - 13.67 (N = 6, p = 0.037) and it was slightly lower in HGG - 12.2 (N = 23, p = 0.037). The average MVD in tumor tissue was the highest in HGG - 20.13 (N = 23, p = 0.047) and other tumors - 18.33 (N = 3, p = 0.047). The remaining types of tumors in the studied group had similar microvessel density – from 13.67 to 14.71.

Antiangiogenic Effects Of Ktp Laser Activated Gold Nanoparticles In Prevention Of Neovascularization

Kareem Esam; Lilian Naoum; Maha Fadel; Mahmoud Saber

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3513-3522

This study examines the antiangiogenic effect of activated naked gold nanoparticles (AuNPS) in dorsal skinfold window chamber rat model after laser coagulation of dorsal blood vessels, to investigate whether the antiangiogenic effect of AuNPs can inhibit reperfusion and neovascularization of photocoagulated blood vessels in an animal model.This study includes 2 groups, each group contains 10 rats, laser group (control group), and AuNPs group. After laser photocoagulation of dorsal blood vessels through the dorsal window chamber fixed on the dorsum of albino rats, the structure of blood vessels and flow dynamics were documented with color digital photography to evaluate photocoagulation and reperfusion. The laser sessions and injection of AuNPs to the epidermal side of the window were twice weekly for 2 weeks. In the laser group (control group), 18 out of 20 photocoagulated blood vessels reperfused within 4-12 days with reperfusion rate 90%. In AuGNPs group 9 out of 22 photocoagulated blood vessels reperfused within 4-12 days with reperfusion rate 40%. Laser activated naked gold nanoparticles have significant antiangiogenic effect, with minimal side effects detected with local use.