Online ISSN: 2515-8260

Keywords : Osteomyelitis

A Study of Aerobic Bacteriological Profile and Antimicrobial Susceptibility Pattern of Isolates in Patients with Chronic Osteomyelitis at Tertiary Care Hospital

Abhishek Binnani, Yogendra Taneja, Prabhakar Kumawat, Bhagirath Ram Bishnoi, B P Sharma, Anjali Gupta, Kirti Shekhawat

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2210-2217

Introduction:  In recent times, there has been change in bacteriological profile of chronic osteomyelitis cases as well as susceptibility of antibiotics.
Objectives: 1. To study Demographic characteristics of Chronic Osteomyelitis cases, 2. To isolate causative Aerobic bacteria (other than Mycobacteria) 3. To analyze Antimicrobial Susceptibility Pattern of isolated aerobic bacteria.
Methodology: A cross sectional hospital-based study of 1 year duration was conducted at Department of Microbiology, S. P. Medical College, Bikaner, Rajasthan. After obtaining informed verbal consent, 110 chronic osteomyelitis cases were recruited to study following nonprobability consecutive sampling. Their pus samples were taken and studied for staining, presence of enzymes, biochemical reactions, fermentation tests, antibiotic susceptibility testing. Obtained results were analysed and presented through tables and diagrams.
Results: 50% cases had S. aureus and 18% had P. aeruginosa in their pus samples. Majority of patients were male and almost 1/3rd cases belonged to 16-30 years age group. S. aureus isolates were sensitive to Cotrimoxazole & Linezolid. Most of the isolate of Klebsiella pneumoniae (83.33%) were sensitive to Meropenem. All isolates of Pseudomonas aeruginosa (100%) were sensitive to Polymyxin B and Colistin C.
Conclusion: Study of bacteriological profile and antimicrobial susceptibility pattern helps significantly in clinical management decisions.

Identification of cultivable and non-cultivable organisms causing intraarticular and bone infections using molecular diagnostic techniques

Deepali J Shetty; Dr Shalini Shenoy Mulki; Dr B N Jagannath Kamath; Dr Sevitha Bhat; Dr Archana Bhat

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 643-651

Introduction: Osteomyelitis is pathologically defined as inflammation of bone and bone marrow due to infection with a microbial pathogen. Septic arthritis is pyogenic infection of the joint either due to direct extension from local tissue infection or more commonly as a result of bacteremia. Microbiological diagnosis in these cases can be made by culture on enriched media and PCR. As PCR detects bacterial DNA, it does not rely on the presence of viable bacteria in the sample for identification. The rapidity of PCR along with its higher sensitivity and specificity enables faster and more accurate diagnosis and treatment

Limited Intra-Medullary Debridement With Medulloscopy And Irrigation-Drainage System for Management of Chronic Osteomyelitis Of Long Bones

Anurag singh; anand singh; nikhil oza; aniruddh dash; a. Ganesh; deepak verma

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 6, Pages 1273-1278

Introduction-Chronic osteomyelitis is a therapeutic challenge and even with proper management it is seldom cured. The usual surgical management involves extensive surgery and prolonged hospitalisation. In cases where only intramedullary variety of infection is present, we can proceed with a less debilitating procedure. In this study our aim is to prove the efficacy of limited intramedullary reaming and medulloscopy with post operative suction-irrigation for managing this kind of osteomyelitis.
Methods and Materials - This study was conducted since June 2018 to May 2020 in which we operated nine patients of medullary chronic osteomyelitis with our technique. The inclusion criteria is Patients with Radiographic / MRI proven Chronic osteomyelitis of medullary variety of Femur or Tibia. Entry points were made at proximal and distal ends of lesion in the bone. The medullary cavity was reamed from proximal and distal points using hand reamers. An arthroscope was used to visualise the medullary cavity for remaining infectious nidus. Post operatively a continuous suction-irrigation system was placed.
Results- Eight patients were followed up for a mean duration of 13 months. At final follow up all the patients were free of symptoms without any pain and discharging sinus.The mean ESR and CRP levels at the time of presentation was 28 and 5.67 respectively and at the final followup was 12 and 0.60 respectively.
Conclusion- Intramedullary debridement using a reamer and arthroscope gives good results as a single staged procedure. With addition of suction-irrigation system it clears off any remaining nidus of infection.