Online ISSN: 2515-8260

Keywords : 3


Synthesis Of Divinyl Ester Of Adipic Acid

Askar B. Parmanov; Suvankul E. Nurmonov; Sherzod Djumagulov; Javohirmirzo Isomiddonov

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 909-920

In this work mono- and divinyl esters of adipic acid with using of homogenno-catalytical vinylation by acetylene of adipic acid in solution of dimethylformamide (DMF) in the presence of zinc acetate as catalyst (10% by mass of adipic acid) and AlCl3•6H2O (10% by mass zinc acetate ) in the temperature range of 80-130 °C have been synthesised. The synthesis of divinyl ester of adipic acid has also been studied using vinyl acetate. The influence of the nature of the catalyst and temperature on the yield of the product was studied. The structure of the synthesized mono- and divinyl esters of adipic acid were confirmed by FT-IR, 1H_NMR, LC/MS and 13C-NMR spectral analysis and some their physical constants were determined

SYNTHESIS OF REMARKABLE SUBSTITUTED ARYL 1,3,4-OXADIAZOLE DERIVATIVES AND THEIR POTENT ANTIINFLAMMATORY AND ANALGESIC ACTIVITIES

Vishal kumar; Satish Kumar Sharma; Rizwan Ahmad

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 4406-4415

The key objective of the current research is to synthesize and analgesic action 5- (2-(2,3-dimethylphenylamino)phenyl)-2-(aryl)- 1,3,4-oxadiazole and to test this activity (VT1- VT8). During this investigation, the condensed methanone {2-[(2,3- dimethylphenyl)amino]phenyl}(hydrazinyloxide), and various aryl-acids in presence of phosphorous oxychloride were synthesized in the present investigations by a sequence of 5-(2- (2,3-dimethylphenylamino)phenyl]-2,3,4-oxadiazols (VT1-VT8). Both synthesized compounds have been tested with 50 mg/kg and 10 mg/kg po respectively for their in vivo antiinflammatory and analgesic activities. Carrageen's mediated acute rat paw oedema model and Eddy's hot plate system were used to analyze the anti-inflammatory and analgesic function of the synthesized compounds. Any substances have been effective at their anti-inflammatory and analgesic activities, according to biological results. Elemental research (C, H, N) and spectral analysis also verified the composition of both substances (IR, 1H NMR and mass spectrometry).

Nicotine mediated activation of Pak1/NFkB cascade in pancreatic cancer cells – A pilot study

Sankar Jagadeeshan; M. Manu Prasad; G. Gejoe; Hemdev Bhoopalan; P. Ashraf; Manjula Sudhakaran; S Shabin Ghouse; Raghunathan Malathi

European Journal of Molecular & Clinical Medicine, 2017, Volume 3, Issue 6, Pages 284-288

Background
Tobacco smoking is a major established risk factor for pancreatic cancer (PC), increasing the incidence up to six fold depending on the duration and intensity of smoking. Nicotine is a key toxin in tobacco and cigarette, which may contribute to development of pancreatitis and PC. Our previous studies revealed an aberrant expression of Pak1 in PC as compared to normal pancreas and its association with cancer progression, tumor angiogenesis, drug resistance and metastasis. Here, we explore a potential link between Pak1 expression and smoking-mediated PC pathogenesis and the use of Pak1 inhibitors to curtail this association. Methods Mia Pa Ca 2 cell line was obtained from NCCS, Pune and grown in the presence and absence of 0.5 μM (0.112 μg/ml) nicotine hemisulphate salt (5 h) and further nicotine exposed cells were treated with Pak1 inhibitor, IPA-3 (1 h). Protein, mRNA and kinase activity of Pak1 were evaluated. Using human pancreatic cancer tissue, mRNA from smokers (n = 10) and non –smokers (n = 10) were assessed for Pak1 expression.
Results
Nicotine significantly enhanced the expression and kinase activity of Pak1, with subsequent activation of NF-κB signalling cascade in cooperation with other pathways, this effect was blocked by IPA-3. Also, it was observed that pharmacological blockage or silencing of α7-nAChR abrogated nicotine mediated activation of Pak1/NF-κB. Additionally, we demonstrated up-regulated Pak1 mRNA expression in tissue sample from smokers compared to non-smokers.
Conclusion
Our findings suggest probable mechanism of action of nicotine through Pak1 signalling on PC pathogenesis and this could be targeted using Pak1 inhibitors for PC treatment.

Simian varicella virus is present in skin tissue of rhesus macaques after experimental reactivation

Miller A; Traina-Dorge V; Blackmon Aa; Wellish Ma; Deharo E; Gilden Da; Mahalingam R

European Journal of Molecular & Clinical Medicine, 2015, Volume 2, Issue 4, Pages -

Varicella zoster virus (VZV) causes varicella (chickenpox), establishes latency in ganglia and reactivates decades later to produce zoster in the elderly. Clinical, pathological, immunological and virological features of simian varicella virus (SVV) infection of primates parallel human VZV infection. Primary SVV infection of primates, cause varicella, after which virus becomes latent in ganglionic neurons and reactivates upon social and environmental stress. Five rhesus macaques were infected intrabronchially with 4.0x105 pfu of SVV. Two weeks later, the monkeys developed varicella rash. Twenty months later four of the monkeys were treated once with a 50 mg/kg of anti-CD4 antibody. All 5 monkeys developed zoster rash, 7–55 days after the treatment. Punch biopsies of the skin rash were analyzed for the presence of SVV antigens by immunohistochemistry and immunofluorescence. SVV ORF 63 protein and glycoproteins gH and L were detected in sweat glands in skin from all 5 monkeys.