CHARACTERIZATION AND OPTIMIZATION OF FERMENTATION CONDITIONS FOR INCREASED PRODUCTION OF L-ASPARAGINASE FROM MARINE FUNGI
European Journal of Molecular & Clinical Medicine,
2021, Volume 8, Issue 3, Pages 1758-1769
AbstractPresent study details the isolation and screening of fungi from marine sediments for the selection of a high potential L-Asparaginase producing strain. The study includes characterization and identification by combination approach and optimization of the process parameters for maximization of L-Asparaginase production by the potential strain. Four fungal strains from twenty three isolates were isolated from different places of marine soils of adavuladeevi which show positive for L-Asparaginase production by producing characteristic pink coloration around the colony. Among isolated fungi tested by plate assay and antibacterial studies, the isolate αAW1- 9 from the marine sediment exhibited the highest zone of diameter (2.5cm) and maximum antibacterial activity against Vibrio cholera (22mm) were considered as the potent strain and were used for further studies. Based on it’s morphological and microscopy characteristics as well as 18S rRNA sequence analysis, the isolate designated as αAW1- 9 were identified as novel Fusarium sporotrichioides strain MT232628. Findings made work hold immense importance for maximum production of L-Asparaginase enzyme after optimization of physico chemical parameters such as optimum incubation period for maximum biomass and L-Asparaginase production of αAW1- 9 were 120h and 48h (1.5g/100ml and 9.87/IU), temperature at 48°C (2.23g/100ml and 39.33/IU), pH 7.0 (2.14mg/100ml and 47.1/IU), lactose (1.92g/100ml and 52.35/IU), yeast extract (2.61mg/100ml and 97.75/IU) were considered to be the ideal conditions. Hence this study opens a new avenue for the researchers and pharmaceutics to pay a wider attention to the enzyme production from marine fungi.
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