• Register
  • Login

European Journal of Molecular & Clinical Medicine

  • Home
  • Browse
    • Current Issue
    • By Issue
    • By Subject
    • Keyword Index
    • Author Index
    • Indexing Databases XML
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Contact Us
Advanced Search

Notice

As part of Open Journals’ initiatives, we create website for scholarly open access journals. If you are responsible for this journal and would like to know more about how to use the editorial system, please visit our website at https://ejournalplus.com or
send us an email to info@ejournalplus.com

We will contact you soon

  1. Home
  2. Volume 2, Issue 2
  3. Authors

Online ISSN: 2515-8260

Volume2, Issue2

Regulation of renal fibrosis by the transforming growth factor beta-1 receptor Alk1

    Carlos Martínez-Salgado Carlos Martínez-Salgado Carlos Martínez-Salgado Carlos Martínez-Salgado Jose M. Muñoz-Felix Jose M. Muñoz-Felix Jose M. Muñoz-Felix Jose M. Muñoz-Felix Nuria Perretta-Tejedor

European Journal of Molecular & Clinical Medicine, 2015, Volume 2, Issue 2, Pages -

  • Show Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract

Tubulointerstitial fibrosis –a common end-stage feature of chronic kidney disease- is characterized by the presence of renal myofibroblasts and excessive accumulation of extracellular matrix proteins (ECM) in the renal tubular interstitium. The cytokine transforming growth factor beta 1 (TGF-β1) promotes myofibroblast activation and ECM proteins expression through intracellular Smads activation, having an important role in renal fibrosis. We have recently reported that the heterozygous disruption of the TGF-β1 receptor activin receptor-like kinase 1 (ALK1) leads to an increase in TGF-β induced renal fibrosis after ureteral obstruction. Thus, we analyzed the effect of ALK1 heterozygosity in TGF-β1 induced signaling and its consequent fibrotic response in mouse embryonic fibroblasts (MEFs). We have analyzed Smad signaling pathaways in ALK1 heterozygous MEFs (ALK1+/-) and their respective controls (ALK1+/+) in basal conditions and after TGF-β1 treatment by Western blot and immunofluorescence. Moreover, we have analyzed collagen I, fibronectin and connective tissue growth factor (CTGF) expression in basal conditions, after TGF-β1 stimulation and after ALK5 inhibition –with SB431542- and Smad3 inhibition –with SIS3-. TGF-β1 stimulation induced Smad2 and Smad3 phosphorylation and Smad2/3 translocation into the nucleus in ALK1+/+ and ALK1+/- MEFs, being this increase higher in ALK1+/- MEFs. Basal Smad2 and Smad3 phosphorylation was higher in ALK1 heterozygous fibroblasts. TGF-β1 stimulation did not induce Smad1 phosphorylation in ALK1+/- MEFs and a very small Smad1 phosphorylation in ALK1+/- MEFs. ALK1 heterozygous MEFs expressed more collagen I, fibronectin and CTGF than their respective controls in basal conditions. Stimulation with TGF-β1 lead to an increase in collagen I, fibronectin and CTGF, being the increase in fibronectin and CTGF higher in ALK1+/- MEFs. Inhibition of ALK5 and Smad3 reversed the ALK1+/- phenotype.
Keywords:
    Summarizing ALK1 heterozygous disruption leads to an impairment of TGF β 1 signaling and increased TGF 1 induced fibrotic response in mouse embryonic fibroblasts
  • PDF (59 K)
  • XML
(2015). Regulation of renal fibrosis by the transforming growth factor beta-1 receptor Alk1. European Journal of Molecular & Clinical Medicine, 2(2), -.
Carlos Martínez-Salgado; Carlos Martínez-Salgado; Carlos Martínez-Salgado; Carlos Martínez-Salgado; Jose M. Muñoz-Felix; Jose M. Muñoz-Felix; Jose M. Muñoz-Felix; Jose M. Muñoz-Felix; Nuria Perretta-Tejedor. "Regulation of renal fibrosis by the transforming growth factor beta-1 receptor Alk1". European Journal of Molecular & Clinical Medicine, 2, 2, 2015, -.
(2015). 'Regulation of renal fibrosis by the transforming growth factor beta-1 receptor Alk1', European Journal of Molecular & Clinical Medicine, 2(2), pp. -.
Regulation of renal fibrosis by the transforming growth factor beta-1 receptor Alk1. European Journal of Molecular & Clinical Medicine, 2015; 2(2): -.
  • RIS
  • EndNote
  • BibTeX
  • APA
  • MLA
  • Harvard
  • Vancouver
  • Article View: 38
  • PDF Download: 73
  • LinkedIn
  • Twitter
  • Facebook
  • Google
  • Telegram
Journal Information

Publisher:

Email:  info@ejmcm.com

  • Home
  • Glossary
  • News
  • Aims and Scope
  • Privacy Policy
  • Sitemap

Editorial Team:  editor@ejmcm.com

For Special Issue Proposal : chiefeditor.ejmcm@gmail.com / info@ejmcm.com

This journal is licensed under a Creative Commons Attribution 4.0 International (CC-BY 4.0)

Powered by eJournalPlus