• Register
  • Login

European Journal of Molecular & Clinical Medicine

  • Home
  • Browse
    • Current Issue
    • By Issue
    • By Subject
    • Keyword Index
    • Author Index
    • Indexing Databases XML
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Contact Us
Advanced Search

Notice

As part of Open Journals’ initiatives, we create website for scholarly open access journals. If you are responsible for this journal and would like to know more about how to use the editorial system, please visit our website at https://ejournalplus.com or
send us an email to info@ejournalplus.com

We will contact you soon

  1. Home
  2. Volume 3, Issue 2
  3. Authors

Online ISSN: 2515-8260

Volume3, Issue2

Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12- dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats

    Krishnamoorthy Deepalakshmi Sankaran Mirunalini

European Journal of Molecular & Clinical Medicine, 2016, Volume 3, Issue 2, Pages 73-82

  • Show Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract

Neoplastic growth of the breast is the most common malignancy in women worldwide and its incidence has increased in most countries. In this study, we have evaluated the efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. (P. ostreatus) an edible mushroom on modulating levels of xenobiotic metabolizing enzymes, hormonal status of estrogen and progesterone receptor (ER/PR), protein expressions and histopathological analysis in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis using a rat model. DMBA was induced by single subcutaneous injection at a dosage of 25 mg in 1 mL vehicle. The ethanolic extract of P. ostreatus (POEet) was administered orally at a concentration of 600 mg/kg bwt as pre- and post-initiation stage of treatment throughout the experimental period which was also compared with standard tamoxifen (TAM) (10 mg/kg bwt). At the end of 16 weeks, our results showed the elevated phase I and depleted phase II metabolizing enzymes, over expression of (ER/PR) and the expression pattern of the proteins such as fas, fasL, caspase 3, caspase 8, caspase 9, Bax were found to be down regulated whereas p53, Bcl2, cox-2 and cyclin D1 were markedly upregulated in DMBA-induced Sprague-Dawley rats, which were significantly reversed on P. ostreatus administration. Moreover, pre-treatment with P. ostreatus showed improved response when compared to that of posttreatment. Based on scientific appraisal, we conclude that the dietary consumption of P. ostreatus might offer maximum protection against DMBA-induced mammary carcinogenesis and improving human health if used as a regular basis.
Keywords:
    Mammary cancerPleurotus ostreatusDetoxification enzymesER/PR statusp53Caspase 8
  • PDF (1707 K)
  • XML
(2016). Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12- dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats. European Journal of Molecular & Clinical Medicine, 3(2), 73-82.
Krishnamoorthy Deepalakshmi; Sankaran Mirunalini. "Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12- dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats". European Journal of Molecular & Clinical Medicine, 3, 2, 2016, 73-82.
(2016). 'Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12- dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats', European Journal of Molecular & Clinical Medicine, 3(2), pp. 73-82.
Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12- dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats. European Journal of Molecular & Clinical Medicine, 2016; 3(2): 73-82.
  • RIS
  • EndNote
  • BibTeX
  • APA
  • MLA
  • Harvard
  • Vancouver
  • Article View: 12
  • PDF Download: 19
  • LinkedIn
  • Twitter
  • Facebook
  • Google
  • Telegram
Journal Information

Publisher:

Email:  info@ejmcm.com

  • Home
  • Glossary
  • News
  • Aims and Scope
  • Privacy Policy
  • Sitemap

Editorial Team:  editor@ejmcm.com

For Special Issue Proposal : chiefeditor.ejmcm@gmail.com / info@ejmcm.com

This journal is licensed under a Creative Commons Attribution 4.0 International (CC-BY 4.0)

Powered by eJournalPlus