EVALUATION OF ANTIBACTERIAL EFFECTIVENESS OF VARIOUS CHEMICAL AND HERBAL ROOT CANAL IRRIGANTS AGAINST ENTEROCOCCUS FAECALIS: AN IN-VITRO STUDY
European Journal of Molecular & Clinical Medicine,
2020, Volume 7, Issue 11, Pages 8845-8856
AbstractA study was conducted to evaluate the antibacterial efficiency of various chemical and herbal root canal irrigants against Enterococcus faecalis. One hundred forty extracted human mandibular premolar teeth were decoronated and biomechanical preparation was done in crown-down technique. 10µl culture suspension of E. faecalis (ATCC 29212) was placed into the prepared root canal space of all teeth. After 48 hours of incubation, all teeth were randomly divided into seven groups with 20 samples per each group. Group A (n=20): Triphala, Group B (n=20):Camellia sinensis (Green tea polyphenols), Group C (n=20): Biopure MTAD solution, Group D (n=20): QMix 2in1 solution, Group E (n=20):2% Chlorhexidine gluconate solution, Group F (n=20):5%Sodium hypochlorite, Group G (n=20):Normal saline (Positive control) were used as root canal irrigants. The time of contact of each irrigant in root canal space was 5 minutes and canals were then flushed with distilled water, excess moisture was removed using paper points .Dentinal shavings were harvested from the walls of root canal space in all samples using Gates-Glidden drills and were transferred into test tubes containing saline, were serially diluted and placed in 140 tryptic soy agar plates, incubated at 37ºC for 48 hours. Colony forming units of E. faecalis were then counted using digital colony counter. Results of the present study showed statistically significant difference among the seven groups in One-way ANOVA test, as P-value was < 0.001. Tukey’s post hoc test showed inter-group comparison between Group A, Group E and Group F were statistically non-significant, as P-value was > 0.05.Based on our study results, we would like to conclude that QMix 2 in1 solution showed maximum antibacterial efficiency on E. faecalis compared to other root canal irrigants. Triphala, 5% sodium hypochlorite and 2% chlorhexidine gluconate solutions exhibited higher and similar antibacterial efficiency against E. faecalis.
Key words: Antibacterial activity, Colony forming units, E. faecalis.
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