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  2. Volume 7, Issue 9
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Online ISSN: 2515-8260

Volume7, Issue9

MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION

    Thanaa R. Abdulrahman Dhefaf R. Mahdi Jabbar S. Hassan

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 254-259

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Abstract

Background: Methicillin resistance Staphylococcus aureus (MRSA) infection related to community has been enhanced through the world. One of the significant cytotoxins elaborated by a few strains of S. aureus is the Panton Valentine leukocidin (PVL), coded by two genes, lukS- PV and lukF-PV which is element of toxin that created pores in the membranes of cells, the role of PVL in the disease process severity of Staphylococcus aureus till known is debated. Amis: This study was planned to detect the Panton Valentine leucocidin (PVL)genes and MRSA isolated from Burn wound infection in community and hospital acquired settings. Material and methods: Cross-sectional study involved 200 patients who presented with burn wounds from third-degree or fourth-degree. The bacteria from all samples were undergone to Cefoxitin disc diffusion testing utilization a 30 μg cefoxitin disc for methicillin resistant determination. Following DNA extraction, conventional polymerase chain reaction (PCR) was used to detect PVL genes. Results: Twenty-eight samples were positive culture for Staph. aureus, of which 19 (67.8%) patients presented with CAI, and 9 (32.1%) belong Hospital acquired infection A total of 23 (82.1%) showed methicillin resistance. Out of 28 isolates, 7(25%) isolates were harboring PVL gene, all of which were Methicillin resistance community acquired infection. None of S. aureus isolates from hospital acquired infection had PVL. Conclusion: This study indicated high prevalence of PVL among community acquired infection MRSA isolates, and the lack of this gene in Hospital acquired infection
Keywords:
    Panton Valentine Leukocidin Methicillin resistance community acquired infection hospital acquired infection
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(2020). MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION. European Journal of Molecular & Clinical Medicine, 7(9), 254-259.
Thanaa R. Abdulrahman; Dhefaf R. Mahdi; Jabbar S. Hassan. "MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION". European Journal of Molecular & Clinical Medicine, 7, 9, 2020, 254-259.
(2020). 'MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION', European Journal of Molecular & Clinical Medicine, 7(9), pp. 254-259.
MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION. European Journal of Molecular & Clinical Medicine, 2020; 7(9): 254-259.
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