European Journal of Molecular & Clinical Medicine

Abstract

The role of an interaction between PLCγ and the proteins during fertilization is still unclear. To recognize whether fertilization includes PLCβ- Cа2+ and PLCγ release mechanisms, GST-SH2 fusion protein was designed and injected into the egg of Asterina miniata sp. In this study, the mixture of SH2-pGEMT-Easy vector was combined with the component cell of DH5α, the results proved the success of SH2 domains insertion into SH2-pGEMT-Easy vector using AmPLCγ plasmid. AmPLCγ was treated with EcoR1 to make a GST-SH2 fusion protein. The designed new SH2 primer including EcoR1 and Xho 1 digestion site and clone them into pGEX vector system, could be directly detect the interaction protein with SH2 domain with an easy purification