European Journal of Molecular & Clinical Medicine

Abstract

This study was carried out in high graduated laboratories in department of biology, college of science / university of Thi-Qar, during the period from December 2019 to October 2021. Gallbladder Undergoing to Cholecystectomy were 200 cases were collected and diagnosed in AL-Hussein teaching, Al-Amel Hospitals and Noor Al- Hussein and Ibn-Al- Baitar laboratories in Thi-Qar Province /Iraq and 50 samples of normal gallbladder from forensic as control during the amount from December 2019 to July 2020 from both sexes and different age. After cholecystectomy is done to gallbladder; it's put in containers which contain Neutral Buffered Formalin 10% for Histopathological classification ;The results of histological study appeared that there were the most common diseases were Cholecystitis in patient were 79.0 %, and in control were 5.6%. While the less common disease were cancer in patient were 4.2 %, and in control were 0.00%. A total of 11 isolates of Salmonella Typhi which identified by conventional biochemical test and API 20- E were subjected to DNA extraction , 50 of DNA extracted from tissue in formalin and 100 of DNA extracted from the formalin-fixe paraffin-embedded gallbladder tissue were subjected to PCR assay for presence of invA, viaB and H1d genes. The prevalence of Salmonella Typhi among patients infected with Cancer, Cholecystitis, Galls stones, the result indicate among 200 patient 26 of them infected with Salmonella Typhi with percentage (13.06%). A positive results have seen in 26(100%) of sample subjected to PCR assay for presence of invA, viaB and H1d genes. Tree genes were selected for Screening of Salmonella Typhi producing toxins by Multiplex PCR; these genes are cdtB ,pltA and pltB . The results indicated all toxin genes were isolated from S. Typhi of patients with gallstones, while non CdtB isolated from bacteria in patients with Cholecystitis and 84,6% of S.Typhi contain pltA and pltB genes