Indirubin-3'-Oxime Eliminates Human Cholangiocarcinoma Through Apoptosis and Cell Cycle-Arrest
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 3, Pages 642-649
AbstractBackground: Cholangiocarcinoma (CCA) is one of the most prevalent diseases in the
South Asia. Failing to determine this disease on time tends to be a great challenge in its
diagnosis. In several human malignancies, indirubin-3'-oxime (I3O) has been found to
decrease cell growth, cause cessation of cell cycle, and cell death.
Objective: The anticancer effects of I3O on human CCA cells will be demonstrated by
exhibiting induced cessation of cell cycle and apoptosis in I3O-treated cells.
Methods: The viability, apoptosis of cells and cessation of cell cycle were all measured.
Analysis using Western blotting, flow cytometry and immunofluorescence.
Results: According to the findings, in CCA cells, I3O treatment inhibits cell growth and
induces cessation of cell cycle as well as caspase-dependent death.
Conclusion: These data imply that I3O, by regulating the cell cycle and generating
apoptosis, could inhibit tumour growth and hence be a viable treatment drug for
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