European Journal of Molecular & Clinical Medicine

Abstract

Background: Diabetes mellitus is a clinical syndrome characterized by hyperglycemia
due to absolute or relative deficiency of insulin. Type 2 diabetes (T2D) occurs due to a
progressive decline in the ability of the pancreas to secrete enough insulin as well as
insulin resistance in insulin target tissues. The pathophysiology of T2D is characterized
by excessive accumulation of ectopic fat in the liver, pancreas, and skeletal muscles,
eventually manifesting as insulin resistance in these tissues and pancreatic beta cell
dysfunction that ultimately leads to hyperglycemia.Metabolic abnormalities such as
dyslipidemia, hyperinsulinemia, or insulin resistanceand obesity play key roles in the
induction and progression of type 2 diabetesmellitus (T2DM). Objectives: To estimate
the levels of serum myeloperoxidase, apolipoprotein B and glycated hemoglobin in type
2 diabetic patients and also in healthy controls, to observe the relationship between
serum MPO with apo B and glycated hemoglobin and also between glycated
haemoglobin with apo B in type 2 diabetic patients and healthy controls.
Materials and Methods: Case control study was done taking 30 cases of type 2 diabetes
mellitus and 30 age and sex matched healthy controls. In all the subjects, concentrations
of HbA1c, serum apo B and serum MPO were estimated. HbA1c was measured by
turbidimetric method and serum Apo B by immune turbidimetric method using semi
auto analyser CHEM 5 Plus. Serum MPO was measured by ELISA method using
ELISA reader.