European Journal of Molecular & Clinical Medicine

Abstract

Introduction: Bacterial presence in urinary tract infections (UTIs) is frequently reported. Several bacterial pathogens especially the members of Entero bacteriacae, Staphylococcus spp., Enterococci, other coliforms and Pseudomonas aeruginosaare commonly associated with urinary tract infections. Assessment of antibiotic resistance in prevailing bacterial flora is a crucial step in context of thein tricacies of developing resistance.
 
Aim of the study: The present study was conducted to isolate and identify the bacterial flora along with antibiogram profiling of the pathogenic isolates against routine antibiotics from patients with urinary tract infection sat HNB Base Hospital, Srinagar, Garhwal.
 
Materials & Methodology: A total of 816urine samples from clinically suspected patients (including 346 males and 470 females) of UTI were studied. Sample collection, Isolation and biochemical identification of aerobic bacteria followed by antibiotic sensitivity profiling of the pathogenic isolates were done using standard protocols.
 
Result & Conclusion of the study: Out of 816 samples collected, 337 bacterial pathogens were isolated. Among Gram-negative pathogens (48.96% of total isolates), Escherichia colialone accounted for 21.06% of the isolates followed by Klebsiellaspp. (10.38%), Acinetobacter spp.(5.34%), Enterobacter spp, Pseudomonas spp.,Proteus spp.(2.67% each), Citrobacterspp (2.07%), Salmonella spp.(1.1%) and Morganella spp.(0.8%). Among Gram-positive cocci (51.03%of total isolates), Enterococcus (25.51%) were more frequently isolated than Coagulase-negative Staphylococcus (CONS) (18.39%), Streptococcus (4.15%) and Staphylococcus aureus(2.96%). Morganella spp. And Enterobacter spp. were the most resistant pathogens among Gram-negative bacterial isolates.Among Gram-positive bacterial isolates, CONS along with Streptococci were the most resistant pathogens.