European Journal of Molecular & Clinical Medicine

Abstract

Introduction: The chronic non-healing wound infections are generally identified with the presence of bacteria in the wounds. Bacterial presence in the wounds is associated with poor healing. Chronic wounds, with their polymicrobial nature, put a significant burden on health budgets worldwide.
 
Aim of the study: The present study was conducted to isolate and identify the bacterial flora along with antibiotic sensitivity profiling of the pathogenic isolates against routine antibiotics from patients with chronic non-healing wound infection sat HNB Base Hospital, Srinagar, Garhwal.
 
Materials & Methodology: A total of 102 specimens (pus, wound exudates, or tissue biopsy) from patients (including 56males and46 females) having chronic non-healing wound infections were studied. Sample collection, Isolation and biochemical identification of aerobic bacteria followed by antibiotic sensitivity profiling of the pathogenic isolates were done using standard protocols.
 
Result & Conclusion of the study: Staphylococcus aureus were the most prevalent bacteria with 24.6 % of all the isolates. Next to these were Coagulase-negative Staphylococci (CONS) with 11.7% followed by Escherichia coli (9.7%), Enterococcus (8.4%), Klebsiella (8.4%), Acinetobacter (6.5%), Micrococci (6.5%), Diphtheroids (5.8%), Citrobacter (3.9%), Pseudomonas (3.9%) (P. aeruginosa were 66.6%, and 33.3% were P. fluorescens), Neisseria (3.2%), Proteus (3.2%), Streptococci (2.6%) (All were S. pyogenes) and Enterobacter (1.2%). Antibiotic sensitivity profiles of Gram-negative bacterial isolates revealed Acinetobacter and Klebsiella being the most resistant pathogens followed by Enterobacter, Citrobacter, Pseudomonas, E. coli, and Proteus. Antibiotic sensitivity profiles of Gram-positive bacterial isolates revealed Enterococcus and CONS being the most resistant pathogens followed by Streptococci and Staphylococcus aureus.
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