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Comparative evaluation of phenotypic method, MALDI-TOF, BD phoenix and vitek-2 systems for species identification of pathogenic yeasts

    Author

    • Kamlesh Rajpal
,

Document Type : Research Article

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Abstract

Background: The introduction of MALDI-TOF MS, BD PHOENIX, and VITEK-2
equipment into the clinical microbiology laboratory has changed the techniques of
fungal identification. This technique can be used to find cryptic species that have
critical susceptibility patterns. The MALDI-TOF MS, BD PHOENIX, and VITEK-2
systems were used to identify clinical strains. Assuming MALDI-TOF MS isolate
identification as standard, discrepancies between three approaches were evaluated.
Finally, these isolates were subjected to in vitro susceptibility testing.
Results: The agreement between the two approaches for 498 yeast isolates was 93.6
percent (32 discrepant isolates). The BD PHOENIXconcordance with VITEK-2 systems
was lower than MALDI-TOF MS. Several of these discordant yeasts had high
antifungal agent MICs.
Conclusions: We discovered the unusual yeasts, including Candida auris, Trichosporon
faecalis and other yeasts. Although not totally satisfying, the performance of the BD
Phoenix system was superior to that of the Vitek 2 system, particularly its performance
with yeast species that are infrequently isolated in the clinical context. Although new
and promising molecular methods such as MALDI-TOF MS are now available, many
clinical microbiology laboratories continue to rely on instruments such as the BD
Phoenix and Vitek 2 for routine identification of fungal agents.

Keywords

  • Comparison
  • MALDI-TOF MS
  • BD PHOENIXsystems
  • VITEK-2 systems
  • unusual yeast
  • Yeast identification
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European Journal of Molecular & Clinical Medicine
Volume 9, Issue 3
February 2022
Page 5625-5631
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  • PDF Download: 195

APA

Rajpal, K. (2022). Comparative evaluation of phenotypic method, MALDI-TOF, BD phoenix and vitek-2 systems for species identification of pathogenic yeasts. European Journal of Molecular & Clinical Medicine, 9(3), 5625-5631.

MLA

Kamlesh Rajpal. "Comparative evaluation of phenotypic method, MALDI-TOF, BD phoenix and vitek-2 systems for species identification of pathogenic yeasts". European Journal of Molecular & Clinical Medicine, 9, 3, 2022, 5625-5631.

HARVARD

Rajpal, K. (2022). 'Comparative evaluation of phenotypic method, MALDI-TOF, BD phoenix and vitek-2 systems for species identification of pathogenic yeasts', European Journal of Molecular & Clinical Medicine, 9(3), pp. 5625-5631.

VANCOUVER

Rajpal, K. Comparative evaluation of phenotypic method, MALDI-TOF, BD phoenix and vitek-2 systems for species identification of pathogenic yeasts. European Journal of Molecular & Clinical Medicine, 2022; 9(3): 5625-5631.

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