European Journal of Molecular & Clinical Medicine

Abstract

Aims:To evaluate the bacteriological profile and antibiotic sensitivity pattern of isolates from blood
culture.
Methods: In all, 610 patients with a suspected blood stream infection were recruited for this research
from the different OPDs, IPDs, and ICUs located within the hospital. Standard microbiological
procedures, including colony character, gram staining, motility testing, and standard biochemical
tests, were used throughout the process of isolating and identifying the organisms. Antimicrobial
susceptibility test Kirby Bauer's disc diffusion technique was used on Mueller Hinton agar (MHA) in
accordance with the recommendations provided by the CLSI to test antimicrobial sensitivity.
Results:During the course of this investigation, a total of 610 blood samples were obtained for the
purpose of blood culture; of them, 120 (19.67 percent) were culture positive. From a total of 120
positive cultures, 49 were gram-positive bacteria (40.83 percent), 64 were gram-negative bacteria
(53.33 percent), and 7 were Candida spp. Klebsiella species, which accounted for 36.37 percent of the
total 120 isolates, was the most prevalent organism, followed by Staphylococcus aureus, which
accounted for 18.33 percent, and Escherichia coli, which accounted for 2.5 percent of the total.
Vancomycin (90.91 percent) and linezolid (90.91 percent) exhibited the greatest activity when
compared to the other antibiotics that were utilised for susceptibility testing for gram-positive isolates.
Conclusion:Klebsiella species was the most prevalent organism, followed by Staphylococcusaureus
as the second most prevalent organism. Imipenem was the medicine that was shown to be the most
effective against gram-negative bacteria, while Vancomycin and Linezolid were found to be the most
effective against gram-positive bacteria.