European Journal of Molecular & Clinical Medicine

Abstract

Background:Mycobacterium tuberculosis was discovered by Robert Koch on March 24, 1882, and every year this day is remembered as the World Tuberculosis (TB) Day.
Objectives: To detect MDR-TB among new cases of smear positive pulmonary tuberculosis by Genotyping method.
Materials and methods: Prospective study was conducted in patients with sputum-positive pulmonary tuberculosis to detect resistance to Rifampicin and Isoniazid using Genotyping by Hain’s technique at RNTCP in IGIMS, Patna , Bihar. 78 patients were study duration of eighteen months. The data was collected after obtaining permission from Institutional Ethical Committee,
Conclusion: LPA- Hain’s technique identifies sensitivity to Rifampicin and Isoniazid in the clinical isolates and provides results in 2-3 days which in turn helps the patient to initiate early treatment and community at large to prevent the spread of MDR-TB.